Double-chamber stirred bioreactor improves the repaired effect of beta-tricalcium phosphate on goat knee cartilage defects

doi:10.3969/j.issn.2095-4344.2014.03.008

Abstract

Abstract:

BACKGROUND: A preliminary experiment developed a double-chamber stirred bioreactor which can carry out osteogenic and cartilage induction at the same time.
OBJECTIVE: To explore the effects of double-chamber stirred bioreactor on the repair of goat knee cartilage defects with tissue-engineered cartilage.
METHODS: Twelve goats were selected to make bilateral femoral condyle osteochondral defects models and randomized to three groups: experimental group, implanted with the composites of β-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor under mechanical stimulation; control group, implanted with the composites of β-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor; blank control group, without treatment. After 12 and 24 weeks of implantation, general observation, Masson staining, II collagen immunohistochemical staining and histological scoring were performed.
RESULTS AND CONCLUSION: In the experimental and control groups, new cartilage tissue and bone tissue were visible, but the experimental group showed better repair effects than the control group (P < 0.05). The blank control group had no cartilage formation. These findings indicate that under the mechanical stimulation by the double-chamber stirred bioreactor in vitro, the repair effect of tissue-engineered osteochondral complex on knee joint cartilage defects can be improved.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, tissue engineering, bioreactors, stem cells, compomers

CLC Number:

R318

Li Jian-wei, Zhao Hui, Zhang Xiao-qiang, Wang Lei, Xia Li-heng, Jin Dan, Wang Gang, Yu Bin. Double-chamber stirred bioreactor improves the repaired effect of beta-tricalcium phosphate on goat knee cartilage defects[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(3): 377-382.

Figures/Tables 3

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