Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (28): 4429-4436.doi: 10.3969/j.issn.2095-4344.2014.28.001
Hao Yao, Qiao Liang, Hao Yong-zhuang, Xiang Chuan
Online:
2014-07-02
Published:
2014-07-02
Contact:
Xiang Chuan, Associate professor, Department of Orthopedics, Second Hospital, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
About author:
Hao Yao, Studying for master’s degree, Department of Orthopedics, Second Hospital, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
CLC Number:
Hao Yao, Qiao Liang, Hao Yong-zhuang, Xiang Chuan. Differentiation of bone marrow mesenchymal stem cells into chondrocyte-like cells under the conditions of transforming growth factor beta and cyclical tensile strain[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(28): 4429-4436.
2.2 各组上清液中糖胺聚糖水平 第1天:周期性拉伸应变组、周期性拉伸应变+转化生长因子β组中糖胺聚糖水平较空白组相比降低(P < 0.05) (表1,图2)。 第3天:转化生长因子β组、周期性拉伸应变组、周期性拉伸应变+转化生长因子β组中糖胺聚糖水平与空白组相比差异均有显著性意义(P < 0.05),其中转化生长因子β组和周期性拉伸应变+转化生长因子β组与空白组相比有上升趋势(表1,图2)。 第6天:转化生长因子β组、周期性拉伸应变组、周期性拉伸应变+转化生长因子β组中糖胺聚糖水平与空白组相比差异均有显著性意义(P < 0.05),其中转化生长因子β组和周期性拉伸应变+转化生长因子β组与空白组相比有上升趋势,空白组、转化生长因子β组上清液中糖胺聚糖水平较第3天均增高(P < 0.05) (表1,图2)。 各组第3天、第6天中糖胺聚糖水平较第1天增高(P < 0.05),且呈逐渐上升趋势(表1,图2)。"
2.3 各组上清液中基质金属蛋白酶13水平 第1天:转化生长因子β组、周期性拉伸应变+转化生长因子β组中基质金属蛋白酶13水平较空白组均降低(P < 0.05)(表2,图3)。 第3天:转化生长因子β组中基质金属蛋白酶13水平较空白组升高(P < 0.05)(表2,图3)。 第6天:周期性拉伸应变组中基质金属蛋白酶13水平较空白组升高(P < 0.05),各组上清液中基质金属蛋白酶13水平较第3天均明显增高(P < 0.05)(表2,图3)。 空白组第3天、第6天基质金属蛋白酶13水平与第1天相比差异有显著性意义(P < 0.05)。转化生长因子β组、周期性拉伸应变组、周期性拉伸应变+转化生长因子β组第6天基质金属蛋白酶13水平较第1天均升高(P < 0.05)(表2,图3)。"
2.5 RT-PCR检测结果 2.5.1 Ⅱ型胶原(Col-Ⅱ)mRNA的表达 第1天:转化生长因子β组、周期性拉伸应变+转化生长因子β组中Ⅱ型胶原mRNA相对表达量较空白组增高 (P < 0.05)(表4,图5)。 第3天:周期性拉伸应变+转化生长因子β组Ⅱ型胶原mRNA相对表达量较空白组增高(P < 0.05)(表4,图5)。 第6天:各实验组Ⅱ型胶原mRNA相对表达量较空白组均增高(P < 0.05)。空白组、转化生长因子β组、周期性拉伸应变+转化生长因子β组中Ⅱ型胶原 mRNA相对表达量分别为较第3天均降低(P < 0.05)(表4,图5)。 各组第3天Ⅱ型胶原mRNA相对表达量分别为较第1天均增高(P < 0.05)。周期性拉伸应变组第6天Ⅱ型胶原mRNA相对表达量为较第1天增高(P < 0.05)(表4,图5)。"
2.5.2 基质金属蛋白酶13 mRNA的表达 第1天:转化生长因子β组、周期性拉伸应变+转化生长因子β组中基质金属蛋白酶13 mRNA相对表达量较空白组均降低(P < 0.05)(表5,图6)。 第3天:转化生长因子β组、周期性拉伸应变+转化生长因子β组中基质金属蛋白酶13 mRNA相对表达量较空白组均降低(P < 0.05)。空白组第3天基质金属蛋白酶13 mRNA相对表达量为较第1天降低(P < 0.05)(表5,图6)。 第6天:各实验组基质金属蛋白酶13 mRNA相对表达量较空白组均降低(P < 0.05),空白组中基质金属蛋白酶13 mRNA相对表达量较第3天升高(P < 0.05)(表5,图6)。"
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