Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (37): 6607-6612.doi: 10.3969/j.issn.2095-4344.2013.37.011

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Deguelin treatment delays embryonic development of zebrafish: A gene chip analysis

Li Wen-hao1,2, Liu Rui-jin2, Wu Xin-rong1, 2   

  1. 1Department of Pharmacy, General Hospital of Guangzhou Military Command of PLA, Guangzhou  510010, Guangdong Province, China
    2School of Light Industry and Food, South China University of Technology, Guangzhou  510641, Guangdong Province, China
  • Received:2012-07-27 Revised:2012-10-13 Online:2013-09-10 Published:2013-09-10
  • Contact: Wu Xin-rong, Chief pharmacist, Doctoral supervisor, Department of Pharmacy, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, Guangdong Province, China; School of Light Industry and Food, South China University of Technology, Guangzhou 510641, Guangdong Province, China gzwxrong@yahoo.com.cngzwxrong@yahoo.com.cn
  • About author:Li Wen-hao★, Studying for master’s degree, School of Light Industry and Food, South China University of Technology, Guangzhou 510641, Guangdong Province, China lwh288@qq.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 10251001002000000*

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Abstract:

BACKGROUND: Anticancer drug deguelin delays the embryonic development of zebrafish, but its exact mechanism is not yet clear.
OBJECTIVE: To explore expression differences in genes and signaling pathways in deguelin-treated zebrafish embryos.
METHODS: Zebrafish embryos were harvested from zebrafish fed according to the Zebrafish Book. Zebrafish embryos at 2-cell stage were selected and divided into two groups: control group treated with dimethyl sulfoxide incubator liquid, and experimental group treated with 0.6 μmol/L deguelin. Total RNA was extracted from the two groups at sphere stage. Then, gene chip technique was used to detect differentially expressed genes in the deguelin-treated zebrafish embryos. Real-time quantitative PCR was employed to validate microarray cluster analysis and pathway analysis to explore the mechanism of action of deguelin.
RESULTS AND CONCLUSION: Chip results showed that 407 genes were upregulated more than three times and 461 genes were downregulated more than three times after deguelin treatment. PCR validation results were consistent with those of the chip. Fourteen pathways were identified by KEGG pathway analysis. Deguelin may play an important role by intervening cell metabolism growth and differentiation.

Key words: derris, zebrafish, embryonic development, signal transduction

CLC Number: