Effects of aqueous extract of Sigesbeckia orientalis on JNK signaling pathway in rats with gouty arthritis

doi:10.3969/j.issn.2095-4344.0617

Abstract

Abstract:

BACKGROUND: Sigesbeckia orientalis containing kirenol, alkaloid and saponins exhibits anti-inflammatory and analgesic properties, improves microcirculation, and effectively controls the inflammatory response in acute gout.
OBJECTIVE: To explore the effect of aqueous extract of Sigesbeckia orientalis on JNK signaling pathway in rats with gouty arthritis.
METHODS: Twenty-four Sprague-Dawley rats were randomly divided into control, model and experimental groups (n=8 per group). Animals in the control and model groups were administrated with normal saline and those in the experimental group were treated with aqueous extract of Sigesbeckia orientalis (2 g/kg) via gavage for 7 days. On day 7, sodiate urate was injected into the knee articular cavity of rats in the model and experimental groups at 1 hour after administration to establish the gouty arthritis models. The control group was given the same volume of normal saline. The pathological changes of synovial tissue were observed by hematoxylin-eosin staining.The serum levels of tumor necrosis factor-α, interleukin-8 and -1β were detected by ELISA. The protein levels of JNK and p-JNK in the synovial tissue were determined by western blot assay. The mRNA expression levels of c-jun, AP-1 were tested by RT-PCR.
RESULTS AND CONCLUSION: Compared with the control group, in the model group, there were inflammatory cell infiltration, synovial cell, fibroblast and small vessel hyperplasia in the synovial tissue. There was an overt decrease in the inflammatory cell infiltration, synovial cell, and fibroblast hyperplasia in the experimental group. The serum levels of tumor necrosis factor-α, interleukin-8 and -1β in the model group were significantly increased, but they were significantly reduced in the experimental group. A significant increase in the protein levels of JNK and p-JNK, and the mRNA expression levels of c-jun, AP-1 in the synovial tissue were found in the model group, which was reversed in the experimental group. These results suggest that the occurrence of gouty arthritis is related to the abnormal activation of JNK signaling pathway, and Sigesbeckia orientalis can significantly improve the symptoms of gouty arthritis via inhibiting JNK signaling pathway.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Arthritis, Synovial Membrane, Drugs, Chinese Herbal, Signal Transduction, Tissue Engineering

CLC Number:

R318

Zheng Chunyu1, Yu Xuefeng2, Chen Shuilin2, Xu Yier3, Han Zhongli4, Sun Guicai2. Effects of aqueous extract of Sigesbeckia orientalis on JNK signaling pathway in rats with gouty arthritis[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(36): 5816-5820.

Figures/Tables 3

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