Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (23): 3708-3715.doi: 10.3969/j.issn.2095-4344.2692
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Yuan Guoqiang1, Qin Yongsheng2, Peng Peng2
Received:
2019-09-04
Revised:
2019-09-09
Accepted:
2019-10-19
Online:
2020-08-18
Published:
2020-07-30
Contact:
Peng Peng, MD, Lecturer, Department of Health Service, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300309, China
About author:
Yuan Guoqiang, Master, Lecturer, Department of Physical Education, Zhengzhou University of Aeronautics, Zhengzhou 450015, Henan Province, China
Supported by:
CLC Number:
Yuan Guoqiang, Qin Yongsheng, Peng Peng. High-intensity interval training for treating pathological cardiac hypertrophy in spontaneously hypertensive rats: effects and mechanisms[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(23): 3708-3715.
2.1 实验动物数量分析 在实验过程中,对照组死亡1只,运动组死亡1只、拒跑2只,统计时予以剔除,因此最终样本量为41只,其中正常血压组15只,对照组14只和运动组12只。 2.2 血压的变化 与正常血压组[收缩压:(118±11) mm Hg,舒张压:(90±9) mm Hg]比较,对照组、运动组收缩压和舒张压增加[对照组收缩压:(181±15) mm Hg,舒张压:(98± 9) mm Hg;运动组收缩压:(149±12) mm Hg,舒张压:(93± 10) mm Hg],差异有显著性意义(P < 0.05);与对照组比较,运动组收缩压降低(P < 0.05),舒张压的变化差异无显著性意义(P > 0.05),提示8周高强度间歇运动能够降低高血压大鼠收缩压,但对舒张压无影响,见图1。 "
2.3 心脏结构与功能的变化 与正常血压组比较,对照组心脏质量指数、左心室质量指数和左心室壁厚度升高(均P < 0.05),左心室舒张末期直径和左心室射血分数降低(均P < 0.05),说明高血压大鼠左室出现心腔缩窄,室壁增厚,即向心性肥大,同时心功能下降。与对照组比较,运动组左心室舒张末期直径和左心室射血分数升高(均 P < 0.05),体质量、心脏质量指数、左心室质量指数和左心室壁厚度的变化差异无显著性意义(均P > 0.05);与正常血压组比较,运动组心脏质量指数、左心室质量指数和左心室壁厚度升高(均P < 0.05),上述结果提示8周高强度间歇运动诱导左室心腔扩张,即离心性肥大,伴随心功能升高,见表1。 "
2.6 心脏肥大信号分子蛋白表达量的变化 与正常血压组比较,对照组CnAβ表达升高(P < 0.05),p-NFATc3/t-NFATc3比值下降(P < 0.05)、PI3-K (p110α)无显著变化(P > 0.05),说明高血压诱导Cn/NFAT信号途径激活,但对PI3-K/Akt通路并无影响。与对照组比较,运动组CnAβ和p-NFATc3/ t-NFATc3比值无显著变化(均P > 0.05),PI3-K(p110α)和p-Akt/t-Akt比值升高(均P < 0.05);与正常血压组比较,运动组CnAβ和p-Akt/t-Akt比值升高(P < 0.05),p-NFATc3/ t-NFATc3比值下降(P < 0.05)、PI3-K(p110α)无显著变化(P > 0.05);结果提示8周高强度间歇运动激活PI3-K/Akt途径,但未对Cn/NFAT通路产生抑制作用,见图5-8。 "
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