Biological changes and mechanism of salvianolic acid B in ovariectomized osteoporosis rat models

doi:10.12307/2022.518

Abstract

Abstract: BACKGROUND: The anti-osteoporotic mechanism of salvianolic acid B is different from traditional anti-bone resorption drugs. It has a multi-target pharmacological effect, which has not yet been fully elucidated.
OBJECTIVE: To study the effect of salvianolic acid B on bone density of femur and lumbar vertebra in rats with osteoporosis, and to explore its anti-osteoporotic mechanism.
METHODS: Totally 36 SPF SD rats aged 6 months were randomly divided into three groups: sham operation group, osteoporosis group, and salvianolic acid B group. Bilateral ovaries were removed and the osteoporosis model was replicated in the osteoporosis group and salvianolic acid B group. In the sham operation group, intact ovaries were kept and part of adipose tissue was removed. At 4 weeks after surgery, 50% salvianolic acid B 25 mg/kg was given by gavage for 6 days per week for 12 consecutive weeks in the salvianolic acid B group. In the other two groups, the same amount of normal saline was given for the same time. At 16 weeks postoperatively, all the rats were sacrificed after anesthesia. The bone density of the right posterior femur and lumbar vertebra was scanned by dual-energy X-ray absorptiometry. The levels of osteoprotegerin and receptor activator of nuclear factor-kB ligand in serum were detected by enzyme-linked immunosorbent assay. The protocols were approved by the Animal Management Committee of Bengbu Medical College (approval No. [2021]255).
RESULTS AND CONCLUSION: (1) The bone mineral density of femur in the osteoporosis group was lower than that in the sham group (P < 0.05). The femoral bone mineral density of salvianolic acid B group was significantly higher than that of osteoporosis group (P > 0.05). The bone mineral density of lumbar vertebrae in osteoporosis group was not significantly different from that in sham operation group (P > 0.05). The lumbar vertebrae bone density of salvianic acid B group was higher than that of osteoporosis group, but the difference was not significant (P > 0.05). (2) Osteoprotegerin level of osteoporosis group was lower than that of sham operation group (P < 0.05). Osteoprotegerin level of salvianolic acid B group was higher than that of osteoporosis group, but the difference was not significant (P > 0.05). The level of receptor activator of nuclear factor-kB ligand of osteoporosis group was significantly higher than that of sham operation group (P < 0.05). The level of receptor activator of nuclear factor-kB ligand of salvianolic acid B group was lower than that of osteoporosis group, but the difference was not significant (P > 0.05). (3) These findings suggest that in the early stage of postmenopausal osteoporosis, bone mineral density of femur decreased significantly, but there was no significant change in bone mineral density of the lumbar spine. At present, it is not believed that salvianolic acid B alone can effectively treat osteoporosis in the short term.

Key words: rats, salvianolic acid B, osteoporosis, osteoprotegerin, receptor activator of nuclear factor-kB ligand

CLC Number:

Wang Dong, Ding Hai, Chang Wenju, Wang Jinzi. Biological changes and mechanism of salvianolic acid B in ovariectomized osteoporosis rat models[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(12): 1927-1930.

Figures/Tables 3

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