Changes of cartilage extracellular matrix and Wnt singling pathway in rabbit models of osteoarthritis after treatment by fire-needle moxibustion at Dubi and Neixiyan

doi:10.3969/j.issn.2095-4344.1139

Abstract

Abstract:

BACKGROUND: Acupuncture and moxibustion can improve the articular symptoms of osteoarthritis and alleviate inflammatory reaction. Fire-needle moxibustion has been reported to treat osteoarthritis, showing exact efficacy, but the underlying mechanism remains unclear.
OBJECTIVE: To clarify the effect of fire-needle moxibustion at Dubi (ST 35) and Neixiyan on the rabbit models of osteoarthritis, and the underlying mechanism.
METHODS: Thirty-two 4-month-old New Zealand rabbits were selected (provided by Shanghai Slac Laboratory Animals Co., Ltd.). There were four groups, including sham operation, osteoarthritis, Western medicine, and fire-needle moxibustion groups. The rabbit knees in the latter three groups were fixed using polymer fixing band to establish the model of knee osteoarthritis. Seven days later, the rabbits were treated by sodium hyaluronate, and fire-needle moxibustion at Dubi and Neixiyan, respectively, for 6 consecutive courses with 3 days in between. The levels of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in serum of rabbits in each group were determined by ELISA after treatment. Safranin-O staining was used for osteoarthritis histological evaluation. Mankin score was used to evaluate osteoarthritis model. Changes of Wnt signaling pathway in cartilage tissues were detected by qualified PCR and western blot assay.
RESULTS AND CONCLUSION: Mankin score and histological staining results showed that the cartilage degradation level in the Western medicine, and fire-needle moxibustion groups was downregulated compared with the osteoarthritis group. After treatment, compared with the osteoarthritis group, the serum levels of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 were significantly decreased and increased in the Western medicine and fire-needle moxibustion groups, respectively (P < 0.05). The levels of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1, and β-catenin mRNA and protein in cartilage in the Western medicine and fire-needle moxibustion groups were significantly lower, higher and lower than those in the osteoarthritis group, respectively. These results indicate that fire-needle moxibustion at Dubi and Neixiyan is a simple and effective method for knee osteoarthritis. The mechanism may be through reducing the abnormally increased metalloproteinase-1 level, regulating ratio of metalloproteinase-1 and tissue inhibitor of metalloproteinase-1, regulating the degradation process of extracellular matrix and affecting the Wnt signaling pathway, thereby alleviating the progression of cartilage degeneration.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Signal Transduction, Matrix Metalloproteinases, Tissue Engineering

CLC Number:

R446

Ao Xiaojing, Miao Mao, Tan Yaqin, Hao Hua. Changes of cartilage extracellular matrix and Wnt singling pathway in rabbit models of osteoarthritis after treatment by fire-needle moxibustion at Dubi and Neixiyan[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(11): 1662-1668.

Figures/Tables 3

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