Supernatant of myocardiocyte induces differentiation of bone marrow-derived mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.36.006

Abstract

Abstract:

BACKGROUND: Culture supernatant containing myocardiocyte has been demonstrated to induce differentiation of bone marrow-derived mesenchymal stem cells into myocardiocyte-like cells. This may associate with some or several cytokines in the culture supernatant.
OBJECTIVE: To explore if the supernatant of myocardiocyte induces bone marrow-derived mesenchymal stem cells to differentiate into myocardiocyte-like cells is associated with the different cytokine content in the supernatant of myocardiocyte.
METHODS: Bone marrow-derived mesenchymal stem cells were isolated and cultured in vitro by the whole bone marrow adherent culture. Cardiocytes were isolated and cultured by enzyme digestion. Bone marrow-derived mesenchymal stem cells (1×10⁸/L) and cardiocytes (1×10⁵/L) were cultured for 72 hours and the supernatant was collected. Hepatocyte growth factor, insulin-like growth factor 1, platelet-derived growth factor, stem cell factor, fibroblast growth factor and vascular endothelial growth factor levels in culture supernatant of bone marrow-derived mesenchymal stem cells and cardiocytes were detected by enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The content of insulin-like growth factor 1, platelet-derived growth factor and fibroblast growth factor in the supernatant of cardiocytes was significantly higher in cardiocytes group compared with bone marrow-derived mesenchymal stem cells group (P < 0.01). Results indicated that insulin-like growth factor 1, platelet-derived growth factor and fibroblast growth factor in the supernatant of cardiocytes may have capability to induce bone marrow-derived mesenchymal stem cells to differentiate into myocardiocyte-like cells, and insulin-like growth factor 1 may serve as the main cytokine.

Key words: stem cells, mesenchymal stem cells, myocytes, cardiac, insulin-like growth factor I, platelet-derived growth factor, hepatocyte growth factor

CLC Number:

R394.2

Li Chun-mei, Wang Xiu-li, Zhu Hai-liang, Wang Jie, Gong Hai-bin. Supernatant of myocardiocyte induces differentiation of bone marrow-derived mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(36): 6417-6422.

Figures/Tables 6

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