Human umbilical cord blood mesenchymal stem cell transplantation for the treatment of hepatic cirrhosis in rats

doi:10.3969/j.issn.2095-4344.2013.19.007

Abstract

Abstract:

BACKGROUND: Studies have shown that cord blood mesenchymal stem cells can be induced into hepatocyte-like cells in a specific environment, and the transplanted cells can exercise the normal function of liver cells.
OBJECTIVE: To establish the rat model of hepatic cirrhosis induced with carbon tetrachloride, and to observe the therapeutic effect of umbilical cord blood mesenchymal stem cell transplantation on a rat model of hepatic cirrhosis.
METHODS: Hepatic cirrhosis models were established through carbon tetrachloride inducing, then 20 rat models of hepatic cirrhosis were randomly divided into two groups. The control group was injected with 0.5 mL normal saline via the tail vein; the treatment group was injected with chloromethyl benzamide labeled umbilical cord blood mesenchymal stem cell suspension, and the number of the stem cells was 1×10⁶. After 2 weeks, all animals were sacrificed. Liver tissues were obtained for the histological observation and the venous blood was collected to detected the liver function indicators.
RESULTS AND CONCLUSION: The liver cells of the hepatic cirrhosis models were loose and cloudy swelling, part of the cells were degenerated and necrosis, the structure of the hepatic lobule was vague, and several pseudolobules with different sizes were formed which meet the diagnostic criteria of hepatic cirrhosis. Compared with the control group, the level of serum albumin in the treatment group was significantly increased (P < 0.05), while the levels of bilirubin and transaminases in the treatment group were significantly decreased (P < 0.05). There were lots of red chloromethyl benzamide labeled umbilical cord blood mesenchymal stem cells in the liver tissue of the treatment group. Human umbilical cord blood mesenchymal stem cells can effectively improve the physiological function of hepatic cirrhosis through tail vein.

Key words: stem cells, stem cell transplantation, umbilical cord blood mesenchymal stem cells, hepatic cirrhosis, albumin, bilirubin, transaminase, liver function, carbon tetrachloride, rats, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Zhan San-hua, Zhang Lu-feng, Yao Wei-min, Yang Ming-hui, Song Xiao-ling, Jiao Hong-liang. Human umbilical cord blood mesenchymal stem cell transplantation for the treatment of hepatic cirrhosis in rats[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.19.007.

Figures/Tables 6

References

[1] Takami T, Terai S, Sakaida I. Advanced therapies using autologous bone marrow cells for chronic liver disease.Discov Med. 2012;14(74):7-12.

[2] Russo FP, Parola M. Stem cells in liver failure.Best Pract Res Clin Gastroenterol. 2012;26(1):35-45.

[3] Takami T, Terai S, Sakaida I. Stem cell therapy in chronic liver disease. Curr Opin Gastroenterol. 2012;28(3):203-208.

[4] Bassiouny AR, Zaky AZ, Abdulmalek SA, et al. Modulation of AP-endonuclease1 levels associated with hepatic cirrhosis in rat model treated with human umbilical cord blood mononuclear stem cells. Int J Clin Exp Pathol. 2011;4(7): 692-707.

[5] Hwang S, Hong HN, Kim HS, et al. Hepatogenic differentiation of mesenchymal stem cells in a rat model of thioacetamide-induced liver cirrhosis. Cell Biol Int. 2012; 36(3):279-288.

[6] Gao B, Bataller R. Alcoholic liver disease: pathogenesis and new therapeutic targets. Gastroenterology. 2011;141(5): 1572-1585.

[7] Zhang Z, Lin H, Shi M, et al. Human umbilical cord mesenchymal stem cells improve liver function and ascites in decompensated liver cirrhosis patients. J Gastroenterol Hepatol. 2012;27(2):112-120.

[8] Wang HJ, Ren L, Wang GS, et al. Xiandai Shengwu Yixue Jinzhan. 2012;12(1):46-48.王海久,任利,汪庚申,等.骨髓间充质干细胞(BMSCs)移植于大鼠肝硬化模型的动物实验研究[J].现代生物医学进展,2012, 12(1): 46-48.

[9] Hwang S, Hong HN, Kim HS, et al. Hepatogenic differentiation of mesenchymal stem cells in a rat model of thioacetamide-induced liver cirrhosis. Cell Biol Int. 2012;36(3): 279-88.

[10] Wang D, Zhang H, Liang J, et al. Effect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model. Clin Exp Med. 2011;11(1):25-32.

[11] Zhong YS, Lin N, Deng MH, et al. Deficient proliferation of bone marrow-derived mesenchymal stem cells in patients with chronic hepatitis B viral infections and cirrhosis of the liver. Dig Dis Sci. 2010;55(2):438-445.

[12] Dai LJ, Li HY, Guan LX, et al. The therapeutic potential of bone marrow-derived mesenchymal stem cells on hepatic cirrhosis. Stem Cell Res. 2009;2(1):16-25.

[13] Tan GS, Xiang XH, Wang Y, et al. Shijie Huaren Xiaohua Zazhi. 2009;17(11): 1074-1078.谭国胜,向贤宏,王于,等.脂肪间质干细胞移植对大鼠肝硬化模型的治疗作用[J].世界华人消化杂志,2009,17(11): 1074-1078.

[14] El-Ansary M, Abdel-Aziz I, Mogawer S, et al. Phase II trial: undifferentiated versus differentiated autologous mesenchymal stem cells transplantation in Egyptian patients with HCV induced liver cirrhosis. Stem Cell Rev. 2012;8(3): 972-981.

[15] Liu Y, Shi ZL, Zhao ZZ, et al. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(10):1837-1840.刘英,施占立,赵宗泽,等.人脐带源间充质干细胞移植改善四氯化碳诱导肝硬化大鼠的肝纤维化[J].中国组织工程研究,2012, 16(10):1837-1840.

[16] Zhang D, Jiang M, Miao D. Transplanted human amniotic membrane-derived mesenchymal stem cells ameliorate carbon tetrachloride-induced liver cirrhosis in mouse. PLoS One. 2011;6(2):e16789.

[17] Jung KH, Uhm YK, Lim YJ, et al. Human umbilical cord blood-derived mesenchymal stem cells improve glucose homeostasis in rats with liver cirrhosis. Int J Oncol. 2011; 39(1):137-143.

[18] Forbes SJ, Newsome PN. New horizons for stem cell therapy in liver disease.J Hepatol. 2012;56(2):496-499.

[19] Jung KH, Uhm YK, Lim YJ, et al. Human umbilical cord blood-derived mesenchymal stem cells improve glucose homeostasis in rats with liver cirrhosis.Int J Oncol. 2011;39(1): 137-143.

[20] Nikeghbalian S, Pournasr B, Aghdami N, et al. Autologous transplantation of bone marrow-derived mononuclear and CD133(+) cells in patients with decompensated cirrhosis.Arch Iran Med. 2011;14(1):12-17.

[21] Chen Z, Qi LZ, Zeng R, et al. Stem cells and hepatic cirrhosis. Panminerva Med. 2010;52(2):149-165.