In vitro rapid culture system to induce the adipogenic differentiation of rat bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.19.002

Abstract

Abstract:

BACKGROUND: The commonly used adipogenic irritants consist of insulin, dexamethasone, 3-isobutyl-1-methylxanthine and indomethacin. The system is not conducive to the study of adipogenesis inhibitory effect due to the disadvantages of multi-processing factors, long induction period and great cytotoxicity.
OBJECTIVE: To develop a rapid culture method to induce adipogenic differentiation of rat bone marrow mesenchymal stem cells.
METHODS: Rat whole bone marrow cells were primary cultured and passaged with trypsin digestion method. The homogeneous mesenchymal stem cells were enriched, and adipogenic differentiation of rat bone marrow mesenchymal stem cells were in vitro induced with thiazolidinediones (rosiglitazone and pioglitazone), the agonists of peroxisome proliferator-activated receptor γ. Glucose-free, low glucose and high glucose Dulbecco's modified Eagle's media were established, and the classic adipogenic irritants were as the positive control. Morphological observation and oil red O staining were performed to observe the differentiated cells at different time points.
RESULTS AND CONCLUSION: Compared with the classic adipogenic irritants, the rosiglitazone and pioglitazone could induce the rat bone marrow mesenchymal stem cells to differentiate into adipocytes, and accumulations of lipid droplets or lipid vesicles and oil red O staining positive cells could be observed after induced with pioglitazone for 48 hours and rosiglitazone for 72 hours. The optional concentration of pioglitazone and rosiglitazone for adipogenic induction was 0.125 mmol/L and 10 μmol/L, respectively. High glucose could enhance the adipogenic differentiation of rat bone marrow mesenchymal stem cells. The culture system with the adipogenic irritants of pioglitazone and rosiglitazone under the high glucose environment can rapidly induce the rat bone marrow mesenchymal stem cells to differentiate into adipocytes.

Key words: stem cells, bone marrow-derived stem cells, adipogenic differentiation, rapid induction, rosiglitazone, pioglitazone, thiazolidinediones, insulin sensitizer, stem cell photographs-containing paper

CLC Number:

R394.2

Shi Xiao-qing, Hua Xian-liang, Wen Guan-mei. In vitro rapid culture system to induce the adipogenic differentiation of rat bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3430-3436.

Figures/Tables 6

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