Inorganic nanoparticles-embedded bioactive carbon nanofibers: Preparation
and performance evaluation

doi:10.3969/j.issn.2095-4344.2013.16.007

Abstract

Abstract:

BACKGROUND: It has been identified that the introduction of β-tricalcium phosphate nanoparticles, which have good osteogenetic activity, into bio-inert carbon nanofibers can significantly improve the biological activity of carbon nanofibers. Furthermore, it has also been reported that bivalent ions doped β-tricalcium phosphate compounds can promote the new bone formation.
OBJECTIVE: To investigate the effect of incorporation of Zn2+, Mg2+ on the fiber morphology and the osteogenetic activity of β-tricalcium phosphate@carbon nanofibers.
METHODS: To prepare Zn2+/Mg2+ doped β-tricalcium phosphate@carbon nanofibers composite nanofibers, electospinning of polyacrylonitrile solution was combined with CaP sol-gel solution by using triethyl phosphate, calcium nitrate, zinc nitrate and/or magnesium nitrate as precursors. The carbon nanofiber composite was obtained by pre-oxidizing and carbonizing the as-electrospun nanofibers. By in vitro co-culture with mouse osteoblast-like cells (MC3T3-E1), the effect of Zn2+/Mg2+ doping into β-tricalcium phosphate@carbon nanofibers on cell behaviors were evaluated via comparison of cell adhesion efficiency, proliferation and morphology.
RESULTS AND CONCLUSION: The obtained Zn2+/Mg2+ doped β-tricalcium phosphate@carbon nanofibers demonstrated uniform and smooth fiber surface with no bead-in-string structure. Inorganic particles in nanoscale could be seen clearly on the fiber surface and throughout the fiber. Determined by element analysis, it was revealed that the composite fibers were mainly composed of carbon, and the calcium element. The mapping of Mg and Zn showed their distribution was even and their amounts were coincident to feed ratios. Compared to non-doping β-tricalcium phosphate@carbon nanofibers, MC3T3-E1 showed enhanced biological behaviors on Zn2+ or Mg2+ doped β-tricalcium phosphate@carbon nanofibers matrixes, like cell adhesion, spreading, and proliferation being promoted. The results suggested that ions doped β-tricalcium phosphate@carbon nanofibers with higher cytocompatibility and bioactivity might be a kind of attractive substrate for bone regeneration.

Key words: biomaterials, nanobiomaterials, carbon nanofibers, beta-tricalcium phosphate, ion doping, sol-gel, electrospinning, MC3T3-E1 osteoblasts, National Natural Science Foundation of China

CLC Number:

R318

Chi Ming-chao, Sui Gang, Yang Qing, Duan Shun, Shi Yu-zhou, Cai Qing, Yang Xiao-ping. Inorganic nanoparticles-embedded bioactive carbon nanofibers: Preparation
and performance evaluation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2899-2904.

Figures/Tables 5

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