Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (26): 4125-4128.doi: 10.3969/j.issn.2095-4344.2762

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Effect of continuous loading pressure on apoptosis and Wnt/beta-catenin signaling pathway in nucleus pulposus cells

Yin Xunlu1, 2, Zhu Liguo1, 3, Feng Minshan1, 3, Yu Jie1, 2, Zhan Jiawen1, 2, Liang Long1, 2, Han Tao1, 2   

  1. 1Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China; 2China Academy of Chinese Medical Sciences, Beijing 100102, China; 3Beijing Key Laboratory of Bone-Setting Technology of Traditional Chinese Medicine, Beijing 100102, China

  • Received:2019-08-08 Revised:2019-08-10 Accepted:2019-10-29 Online:2020-09-18 Published:2020-08-31
  • Contact: Zhu Liguo, MD, Chief physician, Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China; Beijing Key Laboratory of Bone-Setting Technology of Traditional Chinese Medicine, Beijing 100102, China Feng Minshan, MD, Chief physician, Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China; Beijing Key Laboratory of Bone-Setting Technology of Traditional Chinese Medicine, Beijing 100102, China
  • About author:Yin Xunlu, MD, Attending physician, Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China; China Academy of Chinese Medical Sciences, Beijing 100102, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81804120 and 81774330; the Special Project of State Administration of Traditional Chinese Medicine, No. JDZX2015274

Abstract:

BACKGROUND: Previous studies have found that continuous loading pressure can induce intervertebral disc

degeneration by reducing the number of nucleus pulposus cells and reducing the expression of extracellular matrix in the nucleus pulposus. However, its mechanism of action is unclear.

OBJECTIVE: To study the effect of continuous loading pressure on apoptosis and Wnt/β-catenin signaling pathway in nucleus pulposus cells.

METHODS: The in vitro models of rabbit spinal motion segment under loading pressure were established and divided into control group (no treatment), pressure group (3 kg continuous loading pressure) and observation group (3 kg continuous loading pressure + 20 μmol/L SB216763). After 3 days of intervention, the pathological changes of nucleus pulposus cells were detected by hematoxylin-eosin staining, and the expression of caspase-3 and GSK-3β protein in nucleus pulposus tissue was detected by western blot assay.

RESULTS AND CONCLUSION: Compared with the control group, the expression of Caspase-3 and GSK-3β protein increased significantly under the continuous load pressure (P < 0.05). Compared with the pressure group, the expression of Caspase-3 and GSK-3β protein decreased significantly in the observation group (P < 0.05). Pathological examination showed that the number of nucleus pulposus cells in the pressure group was reduced accompanied by morphological changes of the cells. In the observation group, the number of nucleus pulposus cells was relatively large and the cell morphology was relatively regular. In conclusion, continuous loading pressure can regulate Caspase-3 and GSK-3βexpression via Wnt/beta-catenin signaling pathway, and promote the apoptosis of nucleus pulposus cells.

Key words: nucleus pulposus cells, apoptosis, continuous loading pressure, Wnt/β-catenin signaling pathway, spinal motion segment

CLC Number: