Effect of continuous loading pressure on apoptosis and Wnt/beta-catenin signaling pathway in nucleus pulposus cells

doi:10.3969/j.issn.2095-4344.2762

Abstract

Abstract:

BACKGROUND: Previous studies have found that continuous loading pressure can induce intervertebral disc

degeneration by reducing the number of nucleus pulposus cells and reducing the expression of extracellular matrix in the nucleus pulposus. However, its mechanism of action is unclear.

OBJECTIVE: To study the effect of continuous loading pressure on apoptosis and Wnt/β-catenin signaling pathway in nucleus pulposus cells.

METHODS: The in vitro models of rabbit spinal motion segment under loading pressure were established and divided into control group (no treatment), pressure group (3 kg continuous loading pressure) and observation group (3 kg continuous loading pressure + 20 μmol/L SB216763). After 3 days of intervention, the pathological changes of nucleus pulposus cells were detected by hematoxylin-eosin staining, and the expression of caspase-3 and GSK-3β protein in nucleus pulposus tissue was detected by western blot assay.

RESULTS AND CONCLUSION: Compared with the control group, the expression of Caspase-3 and GSK-3β protein increased significantly under the continuous load pressure (P < 0.05). Compared with the pressure group, the expression of Caspase-3 and GSK-3β protein decreased significantly in the observation group (P < 0.05). Pathological examination showed that the number of nucleus pulposus cells in the pressure group was reduced accompanied by morphological changes of the cells. In the observation group, the number of nucleus pulposus cells was relatively large and the cell morphology was relatively regular. In conclusion, continuous loading pressure can regulate Caspase-3 and GSK-3βexpression via Wnt/beta-catenin signaling pathway, and promote the apoptosis of nucleus pulposus cells.

Key words: nucleus pulposus cells, apoptosis, continuous loading pressure, Wnt/β-catenin signaling pathway, spinal motion segment

CLC Number:

Yin Xunlu, Zhu Liguo, Feng Minshan, Yu Jie, Zhan Jiawen, Liang Long, Han Tao. Effect of continuous loading pressure on apoptosis and Wnt/beta-catenin signaling pathway in nucleus pulposus cells[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(26): 4125-4128.

Figures/Tables 3

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