Long-term histological changes of diced cartilage grafts wrapped in advanced platelet-rich fibrin membrane

doi:10.3969/j.issn.2095-4344.2330

Abstract

Abstract:

BACKGROUND: Advanced platelet-rich fibrin has been studied and prepared by scholars. Advanced platelet-rich fibrin has been proven to promote the regeneration and repair of autologous soft and hard tissues through animal experiments.

OBJECTIVE: To prepare advanced platelet-rich fibrin membrane and observe the histological changes of the diced cartilage grafts wrapped in advanced platelet-rich fibrin membrane and autogenous fascia.

METHODS: The following same procedures were carried out in 12 New Zealand rabbits: preparation of advanced platelet-rich fibrin membrane, collection of cartilage tissue of the left ear and the fascia tissue of the right hind limb of the rabbit. There were three types of cartilage grafts: diced cartilage; diced cartilage wrapped with autogenous fascia; diced cartilage grafts wrapped with advanced platelet-rich fibrin membrane. The grafts then were placed into subcutaneous pockets prepared on the backs of the same rabbits. The grafts were removed, and subjected to general observation and histopathologic examination 4 months after surgery.

RESULTS AND CONCLUSION: (1) The thickness of diced cartilage grafts wrapped with autogenous fascia and advanced platelet-rich fibrin membrane was thicker than diced cartilage grafts; the shape of the grafts was more rounded; and the surface was flatter. (2) The chondrocyte survival rate and regeneration potential of diced cartilage grafts wrapped with advanced platelet-rich fibrin membrane were significantly better than the other two grafts (P < 0.05). There was no statistical difference in the other histological parameters among the three types of cartilage grafts. (3) The results show that advanced platelet-rich fibrin membrane can improve the survival rate of diced cartilage and graft shaping and reduce the resorption of diced cartilage after transplantation.

Key words: platelet-rich fibrin">, , cartilage">, , cartilage transplantation">, , autogenous fascia">, , graft resorption">, , protein experiment">, , material

CLC Number:

Yang Yinhui, Yang Fan, Fan Xu, Xu Fengrui, Zheng Liang, Liu Wei, He Mingwu. Long-term histological changes of diced cartilage grafts wrapped in advanced platelet-rich fibrin membrane[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(34): 5486-5492.

Figures/Tables 7

References

[1] DANIEL RK. Diced cartilage grafts in rhinoplasty surgery: current techniques and applications. Plast Reconstr Surg. 2008;122(6): 1883-1891.

[2] CALVERT JW, BRENNER K, DACOSTA-IYER M, et al. Histological analysis of human diced cartilage grafts. Plast Reconstr Surg. 2006;118(1):230-236.

[3] FIRAT C, GURLEK A, AYDN NE. Viability of cartilage grafts in various forms. J Craniofac Surg. 2011;22(5):1666-1670.

[4] OZTURK MAO. Use of diced cartilage grafts wrapped with amniotic membrane in soft tissue augmentation: experimental study. Ann Otol Rhinol Laryngol. 2013;122(1):66-70.

[5] KEMALOĞLU CA, TEKIN Y. A comparison of diced cartilage grafts wrapped in perichondrium versus fascia. Aesthetic Plast Surg. 2014;38(6):1164-1168.

[6] OZTURK M, AYDIN O. Use of diced cartilage grafts wrapped with amniotic membrane in soft tissue augmentation: experimental study. Ann Otol Rhinol Laryngol. 2013;122(1):66-70.

[7] GÜLER İ, BILLUR D, AYDIN S, et al. Efficacy of platelet-rich fibrin matrix on viability of diced cartilage grafts in a rabbit model. Laryngoscope. 2015;125(3):104-111.

[8] GHANAATI S, BOOMS P, ORLOWSKA A, et al. Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol. 2014;40(6):679-689.

[9] LEI L, YU Y, HAN J, et al. Quantification of growth factors in advanced platelet-rich fibrin and concentrated growth factors and their clinical efficiency as adjunctive to the GTR procedure in periodontal intrabony defects. J Periodontol. 2019 doi: 10.1002/JPER.19-0290.

[10] CLARK D, RAJENDRAN Y, PAYDAR S, et al. Advanced platelet-rich fibrin and freeze-dried bone allograft for ridge preservation: a randomized controlled clinical trial. J Periodontol. 2018;89(4):379-387.

[11] KOBAYASHI E, FLUCKIGER L, FUJIOKA-KOBAYASHI M, et al. Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig. 2016;20(9):2353-2360.

[12] JONITZ A, LOCHNER K, TISCHER T, et al. TGF-beta1 and IGF-1 influence the re-differentiation capacity of human chondrocytes in 3D pellet cultures in relation to different oxygen concentrations. Int J Mol Med. 2012;30(3):666-672.

[13] CHEN Y, KE J, LONG X, et al. Insulin-like growth factor-1 boosts the developing process of condylar hyperplasia by stimulating chondrocytes proliferation. Osteoarthritis Cartilage. 2012;20(4): 279-287.

[14] YÜCE E, KÖMERIK N. Potential effects of advanced platelet rich fibrin as a wound-healing accelerator in the management of alveolar osteitis: a randomized clinical trial. Nigerian J Clin Pract. 2019;22(9):1189.

[15] TITIRINLI K, TEKIN U, ATIL F, et al. Evaluation of advanced platelet rich fibrin (a-prf) on bone healing. is it better than old version? A histological animal study. J Biomater Tissue Eng. 2017; 7(6):478-483.

[16] ALHASYIMI AA, PUDYANI PP, ASMARA W, et al. Enhancement of post-orthodontic tooth stability by carbonated hydroxyapatite- incorporated advanced platelet-rich fibrin in rabbits. Orthod Craniofac Res. 2018;21(2):112-118.

[17] TO M, SU C, HIDAKA K, et al. Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation. Anat Sci Int. 2019;94(3):238-244.

[18] KIM HK, CHU LS, KIM JW, et al. The viability of diced cartilage grafts wrapped in autogenous fascia and AlloDerm® in a rabbit model. J Plast Reconstr Aesthet Surg. 2011;64(8):193-200.

[19] DANIEL R. The role of diced cartilage grafts in rhinoplasty. Aesthet Surg J. 2006;26(2):209-213.

[20] KREUTZER C, HOEHNE J, GUBISCH W, et al. Free diced cartilage: a new application of diced cartilage grafts in primary and secondary rhinoplasty. Plast Reconstr Surg. 2017;140(3):461-470.

[21] BRACAGLIA R, TAMBASCO D, D'ETTORRE M, et al. "Nougat graft": diced cartilage graft plus human fibrin glue for contouring and shaping of the nasal dorsum. Plast Reconstr Surg. 2012; 130(5):741-743.

[22] HAREL M, MARGULIS A. Dorsal augmentation with diced cartilage enclosed with temporal fascia in secondary endonasal rhinoplasty. Aesthet Surg J. 2013;33(6):809-816.

[23] TASMAN AJ. Advances in nasal dorsal augmentation with diced cartilage. Curr Opin Otolaryngol Head Neck Surg. 2013;21(4): 365-371.

[24] TABBAL N, TEPPER OM. Diced cartilage versus solid grafts in rhinoplasty. Aesthetic Plast Surg. 2011;35(4):580-581.

[25] KANG GC, WU Y, NAIDU S, et al. Reconstructing a large helical rim defect: synergy of a postauricular perforator flap, diced cartilage, and otoplasty technique. Ann Plast Surg. 2014;72(6):663-665.

[26] EROL OO. The turkish delight: a pliable graft for rhinoplasty. Plast Reconstr Surg. 2000;105(6):2242-2243.

[27] YILMAZ S, ERCOCEN AR, CAN Z, et al. Viability of diced, crushed cartilage grafts and the effects of Surgicel (oxidized regenerated cellulose) on cartilage grafts. Plast Reconstr Surg. 2001;108(4):1054-1062.

[28] BRENNER KA, MCCONNELL MP, EVANS GRD, et al. Survival of diced cartilage grafts: an experimental study. Plast Reconstr Surg. 2006;117(1):105-115.

[29] ORBAY H, TOBITA M, HYAKUSOKU H, et al. Effects of adipose-derived stem cells on improving the viability of diced cartilage grafts. Plast Reconstr Surg. 2012;129(2):369-377.

[30] BRAMOS A, PERRAULT DP, FEDENKO AN, et al. Porcine mesothelium-wrapped diced cartilage grafts for nasal reconstruction. Tissue Engineering Part A. 2018;24(7-8):672-681.

[31] WANG Z, FENG Z, WU G, et al. The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering. Sci Rep. 2016; 6:28126.

[32] 焦志立,谢晓玲,付冬梅,等.改良型富血小板纤维蛋白在兔颅骨诱导成骨中的组织学观察[J].中国组织工程研究,2017,21(14):2208-2214.

[33] CAYMAZ MG, UYANIK LO. Comparison of the effect of advanced platelet-rich fibrin and leukocyte- and platelet-rich fibrin on outcomes after removal of impacted mandibular third molar: a randomized split-mouth study. Niger J Clin Pract. 2019;22(4):546-552.

[34] ANSARIZADEH M, MASHAYEKHAN S, SAADATMAND M. Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane. Int J Biol Macromol. 2019; 125:383-391.

[35] SOTO-PENALOZA D, PENARROCHA-DIAGO M, CERVERA-BALLESTER J, et al. Pain and quality of life after endodontic surgery with or without advanced platelet-rich fibrin membrane application: a randomized clinical trial. Clin Oral Investig. 2019. doi: 10.1007/s00784-019-03033-5.