Correlation of angiopoietin-1 with angiogenesis during scar formation

doi:10.3969/j.issn.2095-4344.2017.32.013

Abstract

Abstract:

BACKGROUND: There are many studies on the correlation between vascular endothelial growth factor and scar, but the correlation between angiopoietin-1 and scar is rarely reported.
OBJECTIVE: To explore the expression of angiopoietin-1 and its correlation with angiogenesis during scar formation.
METHODS: New Zealand white rabbits irrespective of gender were enrolled, and the scar models were established at the ear ventral center. The scar tissue and normal ear ventral tissue were removed at 1, 2, 4, 8, and 12 weeks after epithelialization. The morphology of scar formation was observed by hematoxylin-eosin staining, changes of angiogenesis during scar formation were observed through immunohistochemical staining of CD34, and the expression level of angiopoietin-1 was detected by western blot assay.
RESULTS AND CONCLUSION: The expression level of angiopoietin-1 in the scar tissue was increased firstly, peaked at 2 weeks after epithelialization, then decreased gradually, and the lowest at 12 weeks close to the normal value. The number of microvessels was the highest at 4 weeks after epithelialization and then decreased gradually. The number of mature vessels was on a rise. Number of mature vessels/total number of microvessels tended to be increased. The expression of angiopoietin-1 was correlated negatively with the number of mature vessels, and number of mature vessels/total number of microvessels during scar formation (P < 0.05). To conclude, angiopoietin-1 may play an important role in angiogenesis during scar formation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Angiopoietin-1, Cicatrix, Cytokines, Tissue Engineering

CLC Number:

R318

Wu Zi-han1, Li Gao-feng2. Correlation of angiopoietin-1 with angiogenesis during scar formation[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(32): 5158-5163.

Figures/Tables 3

2.1 实验动物数量分析实验选用新西兰兔9只，实验过程无脱失，全部进入结果分析。 2.2 各时间点兔瘢痕血管生成素1表达 Western-blot凝胶图像扫描图见图1；各时间点血管生成素1表达变化见表1，图2。 2.3 苏木精-伊红染色观察瘢痕的表皮层逐渐增厚，至上皮化后4周左右最厚，基底层较平坦，以后逐渐变薄，角质层逐渐增厚，基底层可见部分表皮突。瘢痕真皮层逐渐增厚，上皮化后4周时最厚，细胞排列密集，呈满天星样均匀分布，均为成纤维细胞，间或散在微血管，此时瘢痕中的微血管数量最多，胶原纤维排列紧密且无序。以后瘢痕真皮层细胞密度进一步降低，但分布均匀，多分布于血管周围，此时微血管数目也减少。至上皮化12周，胶原纤维排列较前疏松且有序。兔瘢痕的大体形态见图3；苏木精-伊红染色见图4，5。 2.4 CD34免疫组织化学染色观察瘢痕中的微血管大多分布在瘢痕真皮浅层。未成熟的微血管表现为内皮细胞肥大，凸向管腔，细胞核呈圆形或椭圆形，管腔被部分阻塞或完全阻塞；成熟的微血管表现为内皮细胞较为扁平，细胞核呈扁平的条状，管腔通畅。计数微血管总数，成熟血管数。将各组各时间点的成熟血管数的均数与各组各时间点的微血管总数的均数两者的比值比较：(成熟血管数/微血管总数)×100%，得到各组各时间点成熟血管数/微血管总数比值。微血管CD34染色见图6，7。 2.4.1 微血管总数的变化各时间微血管总数变化见表1，图8。微血管总数先增高，在创面上皮化后第4周达峰值，然后逐渐降低。 2.4.2 成熟血管数的变化各时间点成熟血管数变化见表1，图9。随时间延长成熟血管数逐渐增高。 2.4.3 成熟血管数/微血管总数比值的变化各时间点成熟血管数/微血管总数比值变化见表1，图10。成熟血管数/微血管总数比值的变化逐渐增高。 2.4.4 血管生成素1表达与成熟血管数的关系血管生成素1表达与成熟血管数的关系呈负相关(r=-0.908，t=-4.337，P < 0.05)，见图11。 2.4.5 血管生成素1表达与成熟血管数/微血管总数比值的关系无干预组血管生成素1表达与成熟血管数/微血管总数比值的关系呈负相关(r=-0.933，t=-4.480，P < 0.05)，图12。"

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