Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (26): 4217-4221.doi: 10.3969/j.issn.2095-4344.2017.26.020

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Biocompatibility of macrophages with quantum dots

Li Chong1, Yan Cheng2, Jin Hui-min2, Wu Shui-yun2, Qiang Ye-tao2, Yan Nan-nan2, Xiao Teng-fei1, Xia Sheng2 
  

  1. 1Kunshan Hospital of Jiangsu University, Kunshan 215300, Jiangsu Province, China; 2Department of Immunology and Immunoassay, Jiangsu University Medical School, Zhenjiang 212013, Jiangsu Province, China
  • Received:2017-07-20 Online:2017-09-18 Published:2017-09-28
  • Contact: Xia Sheng, Professor, Department of Immunology and Immunoassay, Jiangsu University Medical School, Zhenjiang 212013, Jiangsu Province, China
  • About author:Li Chong, Master, Associate chief physician, Kunshan Hospital of Jiangsu University, Kunshan 215300, Jiangsu Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81172834, 31428006, 31570879; Jiangsu University Medical Clinical Science and Technology Development Fund, No. JLY20140048

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Abstract:

BACKGROUND: Compared with the traditional organic fluorescent dyes, quantum dots present good biomarker characteristics. Especially, quantum dots for cell labeling and targeted bioimaging present unique optical properties.
OBJECTIVE: To investigate the biocompatibility of CdSe/ZnS quantum dots with mononuclear macrophages.
METHODS: The macrophages RAW264.7 were inoculated into 96-well plates containing 0, 50, 100 mg/L CdSe/ZnS quantum dots for 1 or 2 hours. Then, the fluorescent signal was detected by flow cytometry. After 0-24 hours of culture, the fluorescence signal intensity of the macrophages cultured with 50 mg/L CdSe/ZnS quantum dots was detected by flow cytometry. After 18 hours of culture, quantitative PCR was used to detect the levels of tumor necrosis factor α and interleukin 1β in macrophages, and macrophage proliferation cell apoptosis were detected by MTT and flow cytometry, respectively.
RESULTS AND CONCLUSION: The fluorescence signal intensity was positively correlated with the mass concentration of CdSe/ZnS quantum dots, and the intensity of the fluorescent signal was increased with the labeling time. After labeling using 50 mg/L CdSe/ZnS quantum dots, the fluorescence signal of macrophages increased continuously with time, and reached the peak at 18 hours. Compared with 0 mg/L quantum dot group, 50, 100mg/L quantum dot groups could significantly promote the expression of tumor necrosis factor α and interleukin 1β in macrophages (P < 0.01 or P < 0.05). The level of tumor necrosis factor α in the 100 mg/L quantum dot group was higher than that in the 50 mg/L quantum dot group (P < 0.01). The expression of interleukin-1β showed no difference between 50 and 100 mg/L quantum dot groups. The cell proliferation in the 50 and 100 mg/L CdSe/ZnS quantum dot groups was significantly higher than that in the     0 mg/L quantum dot group (P < 0.05), but there was no difference between the former two groups. In addition, 50 and 100 mg/L CdSe/ZnS quantum dots had no significant effect on apoptosis of macrophages. To conclude, CdSe/ZnS quantum dots could activate macrophages and promote their proliferation and secretion of inflammatory factors, but did not affect their apoptosis.

Key words: Quantum Dots, Macrophages, Coated Materials, Biocompatible, Tissue Engineering

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