Histological observation on rabbit skull osteoinduction by advanced platelet-rich fibrin

doi:10.3969/j.issn.2095-4344.2017.14.013

Abstract

Abstract:

BACKGROUND: Previous studies have proved platelet-rich fibrin (PRF) with osteoinduction ability, and the centrifugal speed and time to prepare rabbit advanced PRF (A-PRF) with the most similar structure to that of human PRF have been determined.
OBJECTIVE: To observe the histological changes during A-PRF-induced osteogenesis.
METHODS: Thirty Japanese white rabbits were randomly divided into A-PRF and blank control groups (n=15 per group). The full-thickness defect models were established on the rabbit parietal bone, followed by implanted with A-PRF or nothing, respectively. The model rabbits were killed immediately, at 2, 4, 8 and 12 weeks after modeling, to grossly observe the bone formation, and the histological changes in the defect region were observed through hematoxylin-eosin staining, Masson staining and immunohistochemistry.
RESULTS AND CONCLUSION: Unhealed defects were observed in the blank control group. Gross and histological observations showed that the speed, amount and maturity of bone formation in the A-PRF group were significantly better than those in the blank control group immediately, 4, 8 and 12 weeks after modeling (P < 0.05). Our findings suggest that the rabbit skull bone defect is successfully established. A-PRF can induce osteogenesis, and more mature newly born bones appear with time. Additionally, osteoclasts can act with osteoblasts synergically under the A-PRF induction to promote the bone formation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bicompatible Materials, Bone Regeneration, Osteoprotegerin, Tissue Engineering

CLC Number:

R318

Jiao Zhi-li, Xie Xiao-ling, Fu Dong-mei, Zhao Feng, Sun Yong. Histological observation on rabbit skull osteoinduction by advanced platelet-rich fibrin[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(14): 2208-2214.

Figures/Tables 5

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