Adipose-derived stem cells integrated with concentrated growth factors prevent bisphosphonate-related osteonecrosis of the jaws in SD rats

doi:10.3969/j.issn.2095-4344.3529

Abstract

Abstract:

BACKGROUND: Bisphosphonate-related osteonecrosis of jaw may involve invasive dental surgery, infection, jaw injury, immunosuppressive agents and chemotherapeutic drugs, and its potential mechanism and treatment are not clear. Prevention is the main clinical proposition.
OBJECTIVE: To explore the preventive effect of concentrated growth factors integrated with adipose-derived stem cells on bisphosphonate-related osteonecrosis of jaw.

METHODS: Forty Sprague-Dawley rats were randomly assigned to four groups: control group, zoledronic acid group, concentrated growth factor group, and adipose-derived stem cells combined with concentrated growth factor group (n=10 per group). Except the control group, rats in the other three groups were injected with zoledronic acid (80 μg/kg) through the tail vein once a week for 12 consecutive weeks to establish a bisphosphonate-related osteonecrosis of jaw model. Rats in the control group were injected with 0.1 mL of saline through the tail vein. The right mandibular first molar was extracted at the 8th week. In the concentrated growth factor group, 0.5 cm × 0.5 cm concentrated growth factors were implanted immediately after tooth extraction. In the adipose-derived stem cells combined with concentrated growth factor group, 0.5 cm × 0.5 cm concentrated growth factor/adipose-derived stem cell complex was implanted into the extraction socket immediately after tooth extraction. The control group and zoledronic acid group were not treated after tooth extraction. The rats were killed 8 weeks after extraction. The gingival regeneration and bone tissue regeneration around the extraction wound were detected by observation of tooth extraction wound, Micro-CT and hematoxylin-eosin staining.
RESULTS AND CONCLUSION: Compared with other three groups, concentrated growth factor/adipose-derived stem cell complex significantly promoted the healing of soft tissue and new bone formation around tooth extraction, reduced the dead bone formation, and effectively prevented the occurrence of bisphosphonate-related osteonecrosis of jaw.

Key words: stem cells, adipose derived stem cells, bisphosphonates, jaw bone, osteonecrosis, concentrated growth factor, bone regeneration, rats

CLC Number:

Zhang Shengmin, Cao Changhong, Liu Chao. Adipose-derived stem cells integrated with concentrated growth factors prevent bisphosphonate-related osteonecrosis of the jaws in SD rats[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 2982-2987.

Figures/Tables 2

References

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