Neural stem cell transplantation effects on neuronal apoptosis, differentiation and neurobehavior changes in rats with cerebral ischemia

doi:10.3969/j.issn.2095-4344.2017.13.011

Abstract

Abstract:

BACKGROUND: In recent years, neural stem cell transplantation has become a research hotspot in the treatment of brain injuries, such as cerebral ischemia, but the mechanism has not been fully elucidated.
OBJECTIVE: To study the effect of neural stem cell transplantation on neuronal apoptosis and differentiation and neurobehaviors in cerebral ischemia rats.
METHODS: Ninety Sprague-Dawley rats with cerebral ischemia were randomized into model group, phosphate buffer solution (PBS) group and neural stem cell group (n=30 per group). Another 30 rats were selected as sham operation group with vascular exposure but with no occlusion under anesthesia. Thirty normal 30 rats acted as controls with no treatment. Thereafter, neurological deficit scores, cerebral infarction volume, the number of apoptotic nerve cells, BrdU/NeuN and BrdU/GFAP expression were detected at 3, 7, 14 days after modeling.
RESULTS AND CONCLUSION: On the 7th day and 14th day after modeling, the neurological deficit scores, cerebral infarct volume and the number of apoptotic nerve cells in the neural stem cell group were significantly lower than those in the model group and PBS group (P < 0.05), but there was no statistically significant difference between the model group and PBS group (P > 0.05). On the 3rd day, 7th day and 14th day after modeling, the neurological deficit scores and the number of apoptotic nerve cells in the control group and sham operation group were significantly lower than those in the model group, PBS group and neural stem cell group (P < 0.05). On the 3rd day after modeling, the BrdU/NeuN positive cell count in the model, PBS and neural stem cell groups showed no statistically significant differences (P > 0.05); on the 7th day and 14th day after modeling, the BrdU/NeuN positive cell count in the neural stem cell group was significantly higher than that in the model group and PBS group (P < 0.05), but the difference between the model group and PBS group was not statistically significant (P > 0.05). On the 3rd day, 7th day and 14th day after modeling, BrdU/GFAP positive cell count in the neural stem cell group was significantly lower than that in the model group and PBS group (P < 0.05), and the latter two groups had no statistically significant difference (P > 0.05). In summary, neural stem cell transplantation for cerebral ischemia contributes to the neuronal differentiation of endogenous neural stem cells in the subventricular zone of the lateral ventricles, reduce neuronal apoptosis, and ease ischemic damage to the rat neurological function.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Neural Stem Cells, Brain Ischemia, Tissue Engineering

CLC Number:

R394.2

Liu Yi-min, Zhao Yan-yan, Chen Hong-bing. Neural stem cell transplantation effects on neuronal apoptosis, differentiation and neurobehavior changes in rats with cerebral ischemia[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(13): 2029-2035.

Figures/Tables 4

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