Effect of sodium hyaluronate/chitosan nanoparticles on the neovascularization in burned cornea

doi:10.3969/j.issn.2095-4344.2016.52.007

Abstract

Abstract:

BACKGROUND: Chitosan nanoparticles-encapsuled sodium hyaluronate is an effective drug for the burned cornea.
OBJECTIVE: To verify the effect of sodium hyaluronate/chitosan nanoparticles on the neovascularization in burned cornea.
METHODS: Thirty Sprague-Dawley rats were randomly divided into three groups, and the model of burned cornea caused by base was established in the rats of model and experimental groups, followed by respectively treated with 10 μL sodium hyaluronate/chitosan nanoparticle suspension and normal saline, once daily, for consecutive 4 weeks. Rats only given normal saline were used as controls. Four weeks later, the dynamic growth of newly formed blood vessels in the cornea was observed using silt lamp. The levels of tumor necrosis factor-α and interleukin-6 were detected by ELISA, histological changes of the cornea were observed by hematoxylin-eosin staining, and the mRNA expression levels of vascular endothelial growth factor and cyclooxygenase 2 were detected by real-time PCR.
RESULTS AND CONCLUSION: Compared with the control group, the area of the newly formed blood vessel and the levels of tumor necrosis factor-α, vascular endothelial growth factor and cyclooxygenase 2 were significantly increased in the model group (P < 0.05, P < 0.01). In the experimental group, all above indicators were significantly lower than those in the model group (P < 0.05). There were a large number of inflammatory cells and neovascularization in the model group, but only few inflammatory cells in the experimental group. These results show that sodium hyaluronate/chitosan nanoparticles can inhibit the neovascularization in the burned cornea.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Nanostructures, Cornea, Tissue Engineering

CLC Number:

R318

Lu Jing, Wu Shi-ke, Chen Guang, Zhao Yue, Li Dan.

Effect of sodium hyaluronate/chitosan nanoparticles on the neovascularization in burned cornea

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(52): 7803-7808.

Figures/Tables 3

References

[1]何荣军,杨爽,孙培龙,等.海藻酸钠 /壳聚糖微胶囊的制备及其应用研究进展[J].食品与机械,2010,26(2):166-173.

[2]MarcatoPD,Adami LF,de Melo BarbosaR,et al. Development of a Sustained-release System for Nitric Oxide Delivery using Alginate/Chitosan Nanoparticles. CurrNanosci.2013;9(1):1-7.

[3]Douglas KL,Piccirillo CA,Tabrizian M.Effects of alginate in-clusion on the vector properties of chitosan-based nanoparticles.J Control Release. 2006; 115(3):354-361.

[4]李彩凤,陈向红.康普瑞丁磷酸二钠壳聚糖/海藻酸钠微球的制备工艺研究[J].湖南中医药大学学报, 2012,32(10):12-13.

[5]李柱来,许秀枝,陈鹏,等.ZnS-壳聚糖海藻酸钠包裹布洛芬纳米微球的制备和性能研究[J].中国海洋药物杂志, 2010,29(2):16-21.

[6]De S,Robinson D.Polymer relationships during preparation ofchitosan-alginate and poly-L-lysine-alginate nanospheres.J Control Release. 2003;89(1):101-112.

[7]RajaonarivonyM,VauthierC,CouarrazeG,et al. Development of a new carrier made from alginate. JPharm Sci.1993;82(9):912-917.

[8]Silva MS,Cocenza DS,Grillo R,et al.Paraquat-loaded algi-nate/chitosannanoparticles: Preparation, characterization and soilsorption studies.J Hazar Mater. 2011;190(1):366-374.

[9]张雨菲,李友良,姚远,等.壳聚糖纳米银溶液的稳定性及在织物抗菌整理上的应用[J].高等学校化学学报, 2012, 33(8):1860-1865.

[10]鲍文毅,徐晨,宋飞,等.纤维素/壳聚糖共混透明膜的制备及阻隔抗菌性能研究[J].高分子学报,2015,59(1):49-56.

[11]赵江浩,吴年浪.玻璃酸钠滴眼液对轻中度干眼病患者角膜表面规则性的影响[J].海峡药学,2009,21(11):111-113.

[12]辛志坤,霍冬梅,要博.玻璃酸钠在严重眼球破裂伤修复术中的应用[J].航空航天医药,2010,21(4):494-495.

[13]窦晓燕,李林,司马晶,等.角膜裂伤修补并白内障手术中玻璃酸钠的应用[J].眼外伤职业眼病杂志, 2006,28(1):38-40.

[14]幸正茂,刘菲,袁进.角膜碱烧伤的免疫学机制研究进展[J].眼科研究,2010,28(8):796-800.

[15]中华人民共和国科学技术部.关于善待实验动物的指导性意见.2006-09-30.

[16]孙京华,陈艳艳,刘常明,等.b-FGF,VEGF在碱烧伤大鼠角膜中的表达与新生血管的关系[J].眼外伤职业眼病杂志(附眼科手术),2007,29(2):81-84.

[17]邱培瑾,姚克,裘世杰,等.大鼠角膜碱烧伤后碱性成纤维细胞生长因子在角膜中的表达及意义[J].眼科研究, 2002, 20(2):101-104.

[18]邱培瑾,姚克,朱丽君,等.鼠角膜碱烧伤后血管内皮细胞生长因子在其角膜中的表达及意义[J].中华眼科杂志, 2002, 38(5):58-61,74.

[19]颜世龙,梁丹,林妙丽,等.碱烧伤大鼠角膜新生血管模型的初步探索[J].眼科学报,2005,21(4):165-169,172.

[20]闫丽梦.普萘洛尔对小鼠角膜碱烧伤模型中新生血管抑制作用的实验研究[D].南方医科大学,2014.

[21]邱培瑾,姚克,朱丽君,等.鼠角膜碱烧伤后血管内皮细胞生长因子在其角膜中的表达及意义[J].中华眼科杂志, 2002, 38(5):58-61,74.

[22]赵雁之.玻璃酸钠模拟粘蛋白功能和重建生理泪膜的机制研究[D].南昌大学医学院,2013.

[23]万秀玉.含玻璃酸钠海藻糖滴眼液的研究[D].山东大学, 2006.

[24]杜立群,吴欣怡,霍伟.不同生物膜在兔角膜碱烧伤急性期应用的生物学反应[J].眼科新进展, 2006,26(10): 725-728.

[25]邬桂玉,赵敏,计岩.溴芬酸钠/壳聚糖纳米粒蛋白胶胶联羊膜的制备及其对兔角膜新生血管的影响[J].第三军医大学学报,2013,35(6):495-499.

[26]Haidar ZS,Hamdy RC,Tabrizian M.Protein release kinetics for core-shell hybrid nanoparticles based on the layer-by-layer as －sembly of alginate and chitosan on liposomes.Biomaterials.2008;29(9): 1207-1215.

[27]周炼红,胡燕华,徐惠民,等.碱烧伤后抑制角膜新生血管的实验研究-血管内皮生长因子 165反义RNA的作用[J].眼外伤职业眼病杂志,2004,26(4):217-220.

[28]Li WW,Grayson G,Folkman J,et al.Sustained-release endotoxin. A model for inducing corneal neovascularization.Invest Ophthalmol Vis Sci. 1991; 32(11):2906-2911.

[29]Kawamura A,Tatsuguchi A,Ishizaki M,et al.Expression of microsomalprostaglandin e synthase-1 in fibroblasts of rabbit alkali-burnedcorneas.Cornea.2008;27(10): 1156-1163.

[30]Castro MR,Lutz D,Edelman JL.Effect of COX inhibitors on VEGF-induced retinal vascular leakage and experimental corneal and choroidal neovascularization. Exp Eye Res.2004;79(2):275-285.

[31]Kuwano T,Nakao S,Yamamoto H,et al. Cyclooxygenase 2 is a key enzyme for inflammatory cytokine-induced angiogenesis.FASEB J.2004;18(2): 300-310.

[32]Pola R,Gaetani E,Flex A,et al.Comparative analysis of the in vivo angiogenic properties of stable prostacyclin analogs: a possible role for peroxisome proliferator-activated receptors.J Mol Cell Cardiol. 2004; 36(3):363-370.

[33]Saika S,Ikeda K,Yamanaka O,et al.Loss of tumor necrosis factor alpha potentiates transforming growth factor beta-mediated pathogenic tissue response during wound healing.Am J Pathol. 2006;168(6): 1848-1860.