Construction of the femoral artery thrombosis model in rabbits: the arterial elasticity changes before and after thrombolysis

doi:10.3969/j.issn.2095-4344.2016.49.011

Abstract

Abstract:

BACKGROUND: Ultrasonic technology has been extensively applied to evaluate the vascular elasticity changes, but thrombosis and elasticity changes of the proximal thrombus (the artery of proximal heart, 1 cm distance from the thrombosis) after revascularization are rarely reported.
OBJECTIVE: To evaluate the elasticity changes of the unilateral femoral artery of proximal thrombus in rabbits before and after thrombolysis utilizing ultrasonic technology.
METHODS: Models of unilateral femoral artery thrombosis were successfully prepared in 30 New Zealand white rabbits, and then given thrombolytic therapy by targeted microbubbles carrying urokinase under low- frequency ultrasound. The arterial expansion coefficient, compliance coefficient, elastic coefficient, stiffness, pulse wave velocity, augment index, as well as systolic radial strain and radial strain rate before and after thrombosis and after thrombolysis were determined, respectively.
REAULTS AND CONCLUSION: Compared with before thrombosis, the arterial elastic coefficient, stiffness, pulse wave velocity and augment index were significantly increased in contrast to the significantly decreased arterial expansion coefficient, compliance coefficient, systolic radial strain and radial strain rate after thrombosis (P < 0.05). Thrombolytic therapy significantly increased the arterial expansion coefficient, compliance coefficient, systolic radial strain and radial strain rate and eliminated the arterial elastic coefficient, stiffness, pulse wave velocity and augment index (P < 0.05). In addition, the arterial elastic coefficient, stiffness, pulse wave velocity and augment index after complete revascularization were significantly higher than those before thrombosis (P < 0.05), but there were no significant differences in the arterial expansion coefficient, compliance coefficient, systolic radial strain and radial strain rate between two groups. In conclusion, thrombosis contributes to the increased arterial stiffness and elasticity dysfunction, and thrombolytic therapy can, but not completely, improve artery elastic function.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Ultrasonography, Thrombosis, Arteries, Tissue Engineering

CLC Number:

R318

Hu Xiao-mei, Guan Li-na, Alimina•Awen, Mu Yu-ming. Construction of the femoral artery thrombosis model in rabbits: the arterial elasticity changes before and after thrombolysis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(49): 7371-7376.

Figures/Tables 2

2.2 模型创新性/材料、方法的改进既往研究证明FeCl3诱导的血栓模型为混合型血栓，即血栓的组成包括纤维蛋白、激活的血小板以及红细胞，并且联合动脉夹夹闭可显著提高血栓形成率，实验中制栓选择10%浓度的三氯化铁化联合股动脉远端动脉夹夹闭，研究结果显示30只单侧股动脉均成功形成血栓。溶栓方法选择靶向微泡携带尿激酶及RGDS在低频超声助溶下溶栓，既往研究证明，尿激酶和RGDS按1∶1比例配制与靶向微泡结合为最佳，制备的对比剂性质稳定，结合度高，溶栓效果最佳。实验中此方法明显提高了血栓的溶解。 2.3 模型更接近人类或临床实践/模型稳定性该研究方法形成的血栓结构致密，更接近于临床自发性血栓形成的组织学形态特征，有助于以后溶栓实验的科学性和可信性，溶栓过程中未发现其他并发症。 2.4 各组间超声测量弹性参数比较见表1。①血栓形成前与血栓形成后动脉弹性对比，血栓形成后血血栓近段动脉弹性系数、僵硬度、脉搏波传导速度、增强指数增大(P < 0.05)，血栓近段动脉扩张系数、顺应系数、收缩期径向应变及径向应变率减小(P < 0.05)；②血栓完全溶解后与血栓形成前动脉弹性对比；血栓完全溶解后血栓近段动脉弹性系数、僵硬度、脉搏波传导速度、增强指数略增大(P < 0.05)，血栓近段动脉扩张系数、顺应系数、收缩期径向应变及径向应变率较血栓形成前无统计学差异；③血栓形成后与血栓完全溶解后动脉弹性对比。血栓完全溶解后血栓近段动脉弹性系数、僵硬度、脉搏波传导速度、增强指数数值减小(P < 0.05)，血栓近段动脉扩张系数、顺应系数、收缩期径向应变及径向应变率增大(P < 0.05)。"

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