Vascular ultrastructure in atherosclerosis rats after carotid injury under an electron microscope

doi:10.3969/j.issn.2095-4344.2016.49.006

Abstract

Abstract:

BACKGROUND: Animal models of vascular restenosis used to be established in the normal vessels, and study on the healing results limits in the normal artery, not atherosclerosis; thereby, the model cannot accurately simulate the pathological process after percutaneous transluminal coronary angioplasty.
OBJECTIVE: To establish the common carotid catheter injury model in atherosclerosis rats and to study the pathogenesis of restenosis.
METHODS: The balloon catheter injury was performed on the left common carotid artery of atherosclerosis rats with 2F balloon catheter. The dynamic changes of neointimal and media hyperplasia were observed at different time points after surgery. Meanwhile, the ultrastructure of arterial wall was observed using transmission electron microscope.
RESULTS AND CONCLUSION: The neointima formed at 7 days, and reached a peak at 3 months, vascular lumen was seriously narrow, and abundant smooth muscle proliferation could be found in the damaged artery wall. The smooth muscle cells switched from contractile type to synthetic type at the beginning after injury, and most of cells returned to contractile type finally. These findings indicate that 2.0 balloon catheter can successfully establish the carotid injury model in atherosclerosis rats; neointima hyperplasia results in lumen stenosis after injury; furthermore, phenotype transformation, proliferation, and migration of smooth muscle cells play an important role in the process of neointima hyperplasia.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Models, Animal, Coronal Restenosis, Atherosclerosis, Tissue Engineering

CLC Number:

R318

Hu Ping, Chen Yi, Sheng Jing. Vascular ultrastructure in atherosclerosis rats after carotid injury under an electron microscope[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(49): 7334-7340.

Figures/Tables 1

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