ABCE1 silencing enhances sensitivity to beta-eiemene of electrotransferred ovarian cancer stem cells

doi:10.3969/j.issn.2095-4344.2016.28.007

Abstract

Abstract:

BACKGROUND: It is likely to find that ABCE1 plays an important part in the occurrence and progression of cancers through in-depth study.
OBJECTIVE: To investigate the effects of ABCE1 silencing on the proliferation and sensitivity to β-eiemene of ovarian cancer stem cells via electrotransfection.
METHODS: siRNA sequence of ABCE1 was designed and synthesized, and then was electrotransfection into ovarian cancer stem cells. Subsequently, expressions of ABCE1 mRNA and protein in ovarian cancer stem cells after ABCE1 silencing were detected by RT-PCR and western blot assay; the cell cycle was detected using flow cytometry; the sensitivity of ovarian cancer stem cells to β-eiemene after ABCE1 silencing was analyzed by MTT assay and colony-formation assay.
RESULTS AND CONCLUSION: After ABCE1 silencing, expressions of ABCE1 mRNA and protein in ovarian cancer stem cells were significantly reduced. And the cell cycle was arrested in G0/G1 phase, and the number of cells in S phase significantly decreased. Furthermore, the sensitivity of ovarian cancer stem cells to β-eiemene after ABCE1 silencing was significantly enhanced. In conclusion, ABCE1 silencing cannot only significantly inhibit the proliferation of ovarian cancer stem cells, but also enhance the sensitivity of cancer stem cells to β-eiemene.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Ovarian Neoplasms, Neoplastic Stem Cells, Gene Silencing, Tissue Engineering

CLC Number:

R394.2

Wang Jun, Wang Jian-zhong. ABCE1 silencing enhances sensitivity to beta-eiemene of electrotransferred ovarian cancer stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(28): 4149-4154.

Figures/Tables 1

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