Osteogenic efficiency of induced adipose-derived stem cells under Transwell co-cultured condition

doi:10.3969/j.issn.2095-4344.2016.28.008

Abstract

Abstract:

BACKGROUND: Under co-culture conditions, mesenchymal stem cells could regulate osteogenic differentiation and osteogenesis of osteoblasts.
OBJECTIVE: To observe the osteogenic efficiency of osteoblastic precursor cells co-cultured with undifferentiated bone marrow-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or placenta-derived mesenchymal stem cells in mineralization medium.
METHODS: Adipose-derived stem cells were induced in osteogenic differentiation medium for 7 days before being indirectly co-cultured with undifferentiated mesenchymal stem cells isolated from different tissues (bone marrow group, umbilical cord group and placenta group) in Transwell plates. Induced adipose-derived stem cells cultured alone served as control group. At different experimental intervals, quantitative analysis of alkaline phosphatase activity and calcified matrix was preformed to observe the effects of mesenchymal stem cells from different sources on the osteogenic efficiency of induced adipose-derived stem cells.
RESULTS AND CONCLUSION: Expression of alkaline phosphatase was significantly higher in different experimental groups than the control group (P < 0.05), and it was also higher in the bone marrow group than the umbilical cord and placenta groups (P < 0.05). Quantitative analysis of calcified matrix revealed that the experimental groups were significantly higher than the control group (P < 0.05); and in experimental groups, the umbilical cord group was higher than bone marrow group and placenta group(P < 0.05). These findings indicate that the osteogenic efficiency of induced adipose-derived stem cells is improved dramatically under co-culture conditions.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Tissue Engineering, Alkaline Phosphatase

CLC Number:

R394.2

Sun Shi-chen, Dong Teng-zhe, Huang Xin, Zhang Yun-long, Chen Gang. Osteogenic efficiency of induced adipose-derived stem cells under Transwell co-cultured condition [J]. Chinese Journal of Tissue Engineering Research, 2016, 20(28): 4155-4161.

Figures/Tables 1

References

[1] Wu X, Ren J, Li J. Fibrin glue as the cell-delivery vehicle for mesenchymal stromal cells in regenerative medicine. Cytotherapy. 2012;14(5):555-562.
[2] Mizuno H, Tobita M, Uysal AC. Concise review: adipose-derived stem cells as a novel tool for future regenerative medicine. Stem Cells. 2012;30(5): 804-810.
[3] Wen Y, Jiang B, Cui J, et al. Superior osteogenic capacity of different mesenchymal stem cells for bone tissue engineering. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013;116(5):e324-332.
[4] Kou L, Lu XW, Wu MK, et al. The phenotype and tissue-specific nature of multipotent cells derived from human mature adipocytes. Biochem Biophys Res Commun. 2014;444(4):543-548.
[5] Tsuji W, Rubin J, PMarra KG. Adipose-derived stem cells: Implications in tissue regeneration. World J Stem Cells. 2014;6(3):312-321.
[6] Senarath-Yapa K, McArdle A, Renda A, et al. Adipose-derived stem cells: a review of signaling networks governing cell fate and regenerative potential in the context of craniofacial and long bone skeletal repair. International Journal of Molecular Sciences. 2014;15(6):9314-9330.
[7] Glueck M, Gardner O, Czekanska E, et al. Induction of Osteogenic Differentiation in Human Mesenchymal Stem Cells by Crosstalk with Osteoblasts. Biores Open Access. 2015;4(1):121-130.
[8] Schipani E, Wu C, Rankin EB, et al. Regulation of Bone Marrow Angiogenesis by Osteoblasts during Bone Development and Homeostasis. Front Endocrinol (Lausanne). 2013;4(3):85-91.
[9] Fujita K, Xing Q, Khosla S, et al. Mutual enhancement of differentiation of osteoblasts and osteocytes occurs through direct cell-cell contact. J Cell Biochem. 2014; 115(11):2039-2044.
[10] Santos TS, Abuna RP, Castro Raucci LM, et al. Mesenchymal Stem Cells Repress Osteoblast Differentiation Under Osteogenic-Inducing Conditions. J Cell Biochem. 2015;116(12):2896-2902.
[11] Olivares-Navarrete R, Hyzy SL, Hutton DL, et al. Direct and indirect effects of microstructured titanium substrates on the induction of mesenchymal stem cell differentiation towards the osteoblast lineage. Biomaterials. 2010;31(10): 2728-2735.
[12] Rinker TE, Hammoudi TM, Kemp ML, et al. Interactions between mesenchymal stem cells, adipocytes, and osteoblasts in a 3D tri-culture model of hyperglycemic conditions in the bone marrow microenvironment. Integr Biol (Camb). 2014;6(3): 324-337.
[13] Stenderup K, Justesen J, Eriksen EF, et al. Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis. J Bone Miner Res. 2001;16(6):1120-1129.
[14] Dawson JI, Smith JO, Aarvold A, et al. Enhancing the osteogenic efficacy of human bone marrow aspirate: concentrating osteoprogenitors using wave-assisted filtration. Cytotherapy. 2013;15(2):242-252.
[15] 张圣敏,陈刚,王晨星,等.人颊脂垫脂肪干细胞与皮下脂肪干细胞生物学特性的比较[J].中国组织工程研究,2012, 16(32):5941-5945.
[16] Bogdanova A, Berzins U, Nikulshin S, et al. Characterization of human adipose-derived stem cells cultured in autologous serum after subsequent passaging and long term cryopreservation. J Stem Cells. 2014;9(3):135-148.
[17] Rosenberg N, Rosenberg O, Soudry M. Osteoblasts in bone physiology-mini review. Rambam Maimonides Med J. 2012;3(2):e0013-e0020.
[18] Ratisoontorn C, Seto ML, Broughton KM, et al. In vitro differentiation profile of osteoblasts derived from patients with Saethre-Chotzen syndrome. Bone. 2005; 36(4):627-634.
[19] Panepucci RA, Siufi JL, Silva WA Jr., et al. Comparison of gene expression of umbilical cord vein and bone marrow-derived mesenchymal stem cells. Stem cells (Dayton, Ohio). 2004;22(7):1263-1278.
[20] Chen J, Singh K, Mukherjee BB, et al. Developmental expression of osteopontin (OPN) mRNA in rat tissues: evidence for a role for OPN in bone formation and resorption. Matrix (Stuttgart, Germany). 1993;13(2): 113-123.
[21] de Oliveira PT, Zalzal SF, Irie K, et al. Early expression of bone matrix proteins in osteogenic cell cultures. Histochem Cytochem. 2003;51(5):633-641.
[22] Ulrich C, Rolauffs B, Abele H, et al. Low osteogenic differentiation potential of placenta-derived mesenchymal stromal cells correlates with low expression of the transcription factors Runx2 and Twist2. Stem Cells dev. 2013;22(21):2859-2872.
[23] Fan Z X, Lu Y, Deng L, et al. Placenta- versus bone-marrow-derived mesenchymal cells for the repair of segmental bone defects in a rabbit model. FEBS J. 2012;279(13):2455-2465.
[24] Madrigal M, Rao KS, Riordan NH. A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods. J Transl Med. 2014; 12(2): 260-274.
[25] Ando Y, Matsubara K, Ishikawa J, et al. Stem cell-conditioned medium accelerates distraction osteogenesis through multiple regenerative mechanisms. Bone. 2014;61(8):82-90.
[26] Wang KX, Xu LL, Rui YF, et al. The effects of secretion factors from umbilical cord derived mesenchymal stem cells on osteogenic differentiation of mesenchymal stem cells. PLoS One. 2015;10(3):e0120593- e0120609.
[27] Kmiecik G, Spoldi V, Silini A, et al. Current view on osteogenic differentiation potential of mesenchymal stromal cells derived from placental tissues. Stem Cell Rev. 2015;11(4):570-585.
[28] Osathanon T, Nowwarote N, Manokawinchoke J, et al. bFGF and JAGGED1 regulate alkaline phosphatase expression and mineralization in dental tissue-derived mesenchymal stem cells. J Cell Biochem. 2013; 114 (11): 2551-2561.
[29] 杨冰,周慧,樊飞跃,等.Notch信号转导通路在骨形成和重建中的调节作用[J].天津医药,2011,39(2):190-192.
[30] 顾新丰,蒋垚.RUNX2研究进展[J].医学分子生物学杂志, 2006,3(1):52-54.
[31] Ardeshirylajimi A, Soleimani M, Hosseinkhani S, et al. A comparative study of osteogenic differentiation human induced pluripotent stem cells and adipose tissue derived mesenchymal stem cells. Cell J. 2014; 16(3):235-244.