Dynamic expression of Lingo-1 in a model of white matter injury

doi:10.3969/j.issn.2095-4344.2016.27.013

Abstract

Abstract:

BACKGROUND: Lingo-1 has been identified as a negative regulator of oligodendrocyte differentiation and myelination, which may be closely related to the white matter damage, but there is no systematic report on the dynamic changes of Lingo-1 after white matter damage.
OBJECTIVE: To explore the dynamic expression of Lingo-1 at different time points after white matter injury in newborn rats.
METHODS: Seventy-eight Sprague-Dawley rats aged 3 days old were equally and randomly divided into sham operation group and model group. In the model group, models of white matter injury were established by unilateral ligation of the right common carotid artery combined with hypoxia. In the sham operation group, the right common carotid artery was isolated only, without ligation or hypoxia.
RESULTS AND CONCLUSION: At 7 days after model induction, hematoxylin-eosin staining and immunohistochemical staining for myelin basic protein showed that a selective white matter injury was seen at the injury site of a rat model, suggesting successful model establishment. Fluorescent quantitative PCR and western blot assay results demonstrated that the expression levels of Lingo-1 mRNA and protein were significantly up-regulated at 1 day and reached a peak at 7 days post-surgery. After 7 days, above expression was gradually decreased and the up-regulation of Lingo-1 protein lasted to the 28 days post-surgery compared to the sham operation group. These results show that Lingo-1 protein was closely related to the brain white matter injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Myelin Sheath, Anoxia, Tissue Engineering

CLC Number:

R318

Suo Lei, Yang Yin-xiang, Tang Wen-yan, Luan Zuo . Dynamic expression of Lingo-1 in a model of white matter injury[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(27): 4043-4048.

Figures/Tables 1

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