Effects of hollow porous metal prosthesis combined with inducible factors on growth and osteogenic differentiation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2016.25.004

Abstract

Abstract:

BACKGROUND: Bone morphogenetic protein in combination with hollow porous titanium alloy can improve the affinity with surrounding bone tissues.

OBJECTIVE: To observe the effects of bone morphogenetic protein 2 on growth and osteogenic differentiation of bone marrow mesenchymal stem cells cuftured on a hollow porous metal prosthesis scaffold.

METHODS: Passage 3 Sprague-Dawley rat bone marrow mesenchymal stem cells were directly inoculated onto a hollow porous metal prosthesis, and then the scaffold was cultured in DMEM medium containing 0, 0.001, 0.01, 0.06 and 0.1 g/L bone morphogenetic protein 2, respectively. At 6, 12, 24 and 48 hours after inoculation, cell adhesion was detected by MTT assay. Cell osteogenic differentiation was detected by alizarin red staining at 18 days. Besides, Transwell culture was put on the scaffold, and 5x10⁸/L bone marrow mesenchymal stem cells were added into the upper chamber, and DMEM medium containing 0, 0.001, 0.01, 0.06 and 0.1 g/L bone morphogenetic protein 2 were added into the lower chamber to observe cell migration capability after 0, 6, 12, 24 and 48 hours culture.

RESULTS AND CONCLUSION:After 6-48 hours of inoculation, different mass concentrations of bone morphogenetic protein 2 promoted adhesion of bone marrow mesenchymal stem cells in a time-dependent manner. After 18 days of inoculation, bone marrow mesenchymal stem cells induced by different mass concentrations of bone morphogenetic protein 2 changed from fusiform to polygon, and arranged in a multilayer and overlapped form. Numerous calcified nodules could be found, which were stained red by alizarin red. Additionally, within 6-48 hours of culture, bone morphogenetic protein 2 could promote the migration of bone marrow mesenchymal stem cells in a concentration-and time-dependent manner. In conclusion, bone morphogenetic protein 2 can enhance the adhesion, osteogenic differentiation and migration of bone marrow mesenchymal stem cells cultured on the hollow porous metal prosthesis.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Morphogenetic Proteins, Prostheses and Implants, Stem Cells, Tissue Engineering

CLC Number:

R318

Zhao Zi-chun, Li Ling-wei, Cao Zhi-qiang, Li Zhao-wei, Li Chun-liang, Qi Yuan-yuan . Effects of hollow porous metal prosthesis combined with inducible factors on growth and osteogenic differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(25): 3673-3679.

Figures/Tables 5

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