Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (23): 3464-3469.doi: 10.3969/j.issn.2095-4344.2016.23.018

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Overexpression of microRNA-32 weakens the inhibition of polychlorinated biphenyls to the differentiation of P19 cells into cardiomyocytes

Zhang Song-yu, Li Yan, Liu Jiang-bo, Li Gang   

  1. Department of Cardiology, Affiliated Nanyang Hospital of Zhengzhou University, Nanyang 473009, Henan Province, Chin
  • Received:2016-05-03 Online:2016-06-03 Published:2016-06-03
  • About author:Zhang Song-yu, Master, Associate chief physician, Department of Cardiology, Affiliated Nanyang Hospital of Zhengzhou University, Nanyang 473009, Henan Province, China

Abstract:

BACKGROUND: Polychlorinated biphenyls inhibit the differentiation of P19 cells into cardiomyocytes. In the meanwhile, microRNAs play an important role in regulating cell differentiation.
OBJECTIVE: To explore the effect of microRNA-32(miR-32) on the polychlorinated biphenyls-inhibited differentiation of P19 cells into cardiomyocytes.
METHODS: P19 cells were co-cultured with 2.5 μmol/L polychlorinated biphenyls and 1% dimethyl sulfoxide. Afterwards, α-actinin, desmin, cTnI and GATA4 were identified by western blot assay. Wddwxrof polychlorinated biphenyls on the expression of miR-32 was evaluated by real-time PCR. Mouse eukaryotic vector expressing miR-32 was constructed by gene recombination technology, and transfected into P19 cells followed by co-cultured with 2.5 μmol/L polychlorinated biphenyls and 1% dimethyl sulfoxide. Then, expressions of differentiation-related proteins were detected by western blot assay.
RESULTS AND CONCLUSION: Polychlorinated biphenyls inhibited the differentiation of P19 cells into cardio myocytes and decreased the miR-32 expression. Cell lines overexpressing miR-32 was successfully established in mice. Furthermore, we found that overexpression of miR-32 weakens the inhibition of polychlorinated biphenyls to the differentiation of P19 cells into cardiomyocytes.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: MicroRNAs, Polychlorinated Biphenyls, Myocytes, Cardiac, Cell Differentiation, Tissue Engineering

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