Induced differentiation of human embryonic stem cells into endothelial cells

doi:10.3969/j.issn.2095-4344.2016.23.007

Abstract

Abstract:

BACKGROUND: Human embryonic stem cells exhibit self-renewal and multi-differentiation potential, and can differentiate into endothelial cells under certain induction conditions.
OBJECTIVE: To explore induced conditions of the human embryonic stem cells differentiating into endothelial cells and to investigate the effect of vascular endothelial growth factors on the endothelial differentiation of human embryonic stem cells.
METHODS: After resuscitation, passage 40 human embryonic stem cell lines H9 were subjected to suspension culture to prepare embryos, and after 5-day culture, these cells were cultured in attachment medium to differentiate into embryoid bodies, followed by induction with 50 µg/L vascular endothelial growth factors. Passage 2 and 15 embryonic stem cells after induced differentiation were taken for Dil-Ac-LDL uptake test and immunohistochemical staining, respectively.
RESULTS AND CONCLUSION: After 1-day culture, cord-like or polygonal monolayer cells around embryoid bodies showed bud-like and radial growth with a relative rapid speed merging into surrounding colonies; at 2-3 days, the number of suspension cells increased further, but the small-round cells in the center began to die; at 5 days, embryoid bodies started to passage, and aggregated cells exhibited typical paving stone-like appearance. Moreover, some human embryonic cells after induced differentiation could actively take up fluorescent labeled LDL, and red fluorescent particles appeared. Additionally, passage 15 embryonic stem cells after induced differentiation could express CD31 and FLK-1. These findings suggest that human embryonic stem cells induced by vascular endothelial growth factors can differentiate into endothelial cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Embryonic Stem Cells, Endothelial Cells, Tissue Engineering

CLC Number:

R394.2

Shi Yang, Shen Jun-sheng, Wu Tao-tao, Li Xiao-fei. Induced differentiation of human embryonic stem cells into endothelial cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(23): 3394-3399.

Figures/Tables 2

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