Preparation of the small intestinal submucosa sponge

doi:10.3969/j.issn.2095-4344.2016.21.010

Abstract

Abstract:

BACKGROUND: Studies have found that small intestinal submucosa that is directly implanted into the lesion cannot effectively promote cell growth and differentiation in vivo and in vitro, because of its small pore size and poor permeability.

OBJECTIVE: To establish the small intestinal submucosa sponge and to explore its morphological characteristics.

METHODS: Porcine small intestinal submucosa was prepared by physiochemical method. Then the small intestinal submucosa with the mass fraction of 1%, 2%, 3% and 4% was cross-linked by 50, 100 and 150 mmol/L 1-ehyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, respectively, so as to obtain small intestinal submucosa sponge, whose morphology was detected by lighting and scanning electron microscope. In the meanwhile, small intestinal submucosa as control group, and small intestinal submucosa sponge as test group were intramuscularly implanted into the back of rats, respectively. At 1, 2 and 3 weeks after implantation, histological changes and implant degradation were observed by hematoxylin-eosin staining.

RESULTS AND CONCLUSION: The small intestinal submucosa sponge, which was prepared by the small intestinal submucosa with the mass fraction of 1% and 100 mmol/L cross-linking agent, had elastic and close space structure, uniform pore size and regular structure, so it was selected as the implant into the muscle. At 1 week after implantation, in the test group, the mesh sponge had the complete structure with few neutrophils, lymphocytes and giant cell reaction, and soft tissue hyperplasia and migration surrounding the implant appeared; in the control group, there were numerous inflammatory cells, and wound adhesion and little migration of surrounding tissues could be found. At 3 weeks, inflammatory cells mostly disappeared, and fibroblast-like cells and vascular components appeared, with thinner and regular collagen fiber bundles, and connective tissue-like structures could be found. In contrast, the control group still had numerous inflammatory cells and few collagen fibers. In conclusion, small intestinal submucosa sponge is a potential material used as the tissue-engineered skin scaffold.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biocompatible Materials, Cross-linking Reagents, Tissue Engineering

CLC Number:

R318

Sun Hui-zhe, Tian Wei, Zeng Liang, Wang Xiao-jie, Wang Zheng-dong, Ren Yue, Kuang Bao-ping . Preparation of the small intestinal submucosa sponge[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(21): 3110-3116.

Figures/Tables 7

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