Acellular amniotic membrane versus medical membrane to prevent tendon adhesion in tendon sheath repair

doi:10.3969/j.issn.2095-4344.2016.21.011

Abstract

Abstract:

BACKGROUND: Experiments have demonstrated that biological membranes can be used to reconstruct the tendon sheath and inhibit exogenous healing of the tendon. Therefore, these membranes provide a good bed for tendon gliding and reduce tendon adhesion.

OBJECTIVE: To compare the effects of acellular amniotic membrane and medical membrane against tendon adhesion during the repair of tendon sheath defects.

METHODS: Toes III from the bipeds of 66 leghorns were chosen to prepare tendon injury and tendon sheath defect models, which were randomly divided into three groups (n=22 per group). Amnion group were repaired with acellular amniotic membrane, medical membrane group with absorbable membrane, and control group had no treatment on tendon sheath defects. Gross, histological and biomechanical tests of each group were performed at 2, 4, 8, 12 weeks after surgery.

RESULTS AND CONCLUSION: At 12 weeks after surgery, in the amniotic membrane and medical membrane groups, the tendon sheath formed completely, and the tendon healed well, with no adhesion, but in the control group, there was serious tendon adhesion. At 8 weeks after surgery, the number of synovial cells in the false sheath was highest in the amniotic membrane group sequentially followed by the medical membrane group and control group. In the amniotic membrane group, the rough endoplasmic reticulum expanded highly and secreted exuberantly in the matrix, while in the control group, the synovial cells presented with messy arrangement, and expanded vacuoles in the matrix were weaker than those in the other two groups. At 12 weeks after surgery, fibroblasts were arrayed tidily in layer with dense structure in the medical membrane and amniotic membrane groups; but in the control group, fibroblasts were distributed disorderly with loose structure. Tendon sliding distance and total flexor toe angle in the amniotic membrane and medical film groups were significantly larger than those in the control group (P < 0.05), but there was no significant difference between the medical membrane and amniotic membrane groups. Additionally, the maximum tensile fracture strength had no significant difference among three groups at 12 weeks after surgery. These results indicate that both amniotic membrane and medical membrane can markedly protect the tendon from exogenous healing and adhesion.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Amnion, Tissue Adhesions, Tissue Engineering

CLC Number:

R318

Liu Guo-li, Yu Kun-lun, Bai Jiang-bo, Ma Tao, Yang Yan-tao, Tian De-hu. Acellular amniotic membrane versus medical membrane to prevent tendon adhesion in tendon sheath repair[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(21): 3117-3123.

Figures/Tables 9

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