Biocompatibility of vancomycin/hydroxyapatite/titanium nanotubes

doi:10.3969/j.issn.2095-4344.2016.21.008

Abstract

Abstract:

BACKGROUND: In order to overcome the shortcomings of single materials, antibiotics-loaded hydroxyapatite/titanium composites have attracted people’s attentions.

OBJECTIVE: To evaluate the biocompatibility of vancomycin/hydroxyapatite/titanium nanotubes.

METHODS: Mouse osteoblasts, MC-3T3-E1, were co-cultured with titanium (Cp-T), TiO₂ nanotubes, and vancomycin/hydroxyapatite/titanium nanotubes, respectively. Cell morphology and growth were observed after 1, 3 and 5 days of co-culture under inverted microscope and scanning electron microscope. The cell proliferation was detected by AO-EB method. The total protein, calcium and alkaline phosphatase levels were detected at 7 and 14 days of co-culture.

RESULTS AND CONCLUSION: The MC-3T3-E1 cells with good viability and morphology adhered well on the surface of vancomycin/hydroxyapatite/titanium nanotubes compared with those on the surface of pure titanium and TiO₂ nanotubes under the scanning electron miscroscope. Moreover, there were a large amount of pseudopodia on the surface of composite nanotubes. Compared with the other two groups, the cell number on the surface and the levels of intracellular calcium and alkaline phosphatase were all higher in the vancomycin/hydroxyapatite/titanium nanotubes group. These findings suggest that the vancomycin/hydroxyapatite/titanium nanotubes have good biocompatibility and no cytotoxicity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Vancomycin, Durapatite, Nanotubes, Tissue Engineering

CLC Number:

R318

Zhang Hang-zhou, Tian Ang, Liang Qing-wei, Bai Xi-zhuang, Xue Xiang-xin. Biocompatibility of vancomycin/hydroxyapatite/titanium nanotubes[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(21): 3097-3103.

Figures/Tables 6

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