Acellular embryoid bodies in mice: preparation and effect of promoting differentiation of Lewis lung carcinoma cells

doi:10.3969/j.issn.2095-4344.2016.20.012

Abstract

Abstract:

BACKGROUND: Co-culture with embryonic stem cells or embryonic tissues can induce differentiation of carcinoma cells into normal epithelial cells or decrease malignancy of carcinoma cells. Acellular embryoid bodies retain the structure and important cytokines of embryonic tissues.
OBJECTIVE: To prepare acellular embryoid bodies from mouse embryonic stem cells and to investigate their effects on differentiation of mouse Lewis lung carcinoma cells at three-dimensional culture in vitro.
METHODS: Mouse embryonic stem cells (D3) were dynamically cultured for 7 days to produce embryoid bodies followed by decellularization with 0.1% sodium dodecyl sulfate. Mouse Lewis lung carcinoma cells were co-cultured with acellular embryoid bodies as test group or cultured in three-dimensional matrigel medium for 7 days as control group, respectively. Cell proliferation and expression of E-cadherin were detected by immunohistochemical staining and western blot assay, respectively. In addition, mRNA expressions of Slug and E-cadherin were observed using RT-PCR technology.
RESULTS AND CONCLUSION: Uniform mouse embryoid bodies were successfully prepared, and were completely decellularized with sodium dodecyl sulfate. After 7-day three-dimensional matrigel culture, in the control group, multicellular tumor spheroids were formed, accompanied by a higher Ki67 positive rate; Lewis lung carcinoma cells in the test group were repopulated in the acellular embryoid bodies showing significantly lower Ki67 positive rate. Compared with the control group, the absorbance of Paxillin in the test group was significantly smaller, and the absorbance of E-cadherin was significantly higher (P < 0.05). Besides, mRNA expressions of Slug and E-cadherin were significantly decreased and increased in the test group compared with the control group, respectively (P < 0.05). These findings indicate that the acellular embryoid bodies can promote differentiation of mouse Lewis lung carcinoma cells in three-dimensional culture in vitro.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；
ORCID: 组织工程 0000-0003-2685-4218(吕卫东)

Key words: Embryoid Bodies, Extracellular Matrix, Carcinoma, Lewis Lung, Cell Line, Tumor, Tissue Engineering

CLC Number:

R318

Lv Wei-dong, Cai Lin, Zhang Jia-dong, Lei Guang-yan, Liu Zhi-gang, Zhang Xin-wei, Lu Jian-rong. Acellular embryoid bodies in mice: preparation and effect of promoting differentiation of Lewis lung carcinoma cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(20): 2972-2978.

Figures/Tables 1

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