Table of Content

13 May 2016, Volume 20 Issue 20 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Three-dimensional digital model measurements of the temporomandibular joint Shen Xiao-ling, Liu Min, Huang Hai-xia, Wang Pin 2016, 20 (20):  2893-2898.  doi: 10.3969/j.issn.2095-4344.2016.20.001 Abstract ( 438 )   PDF (1058KB) ( 386 )   Save BACKGROUND: Guidances for design of Chinese people’s temporomandibular joint prosthesis are urgently needed because foreign prosthesis is unsuitable for Chinese people due to variant anatomy parameters. OBJECTIVE: To provide a reference for improving the design of artificial temporomandibular joint through computer three-dimensional digital measures of the anatomy parameters of the temporomandibular joint. METHODS: Continuous CT data with layer thick 0.625 mm of 30 normal temporomandibular joints were selected. Three-dimensional digital reconstruction and eight related anatomy parameter measurements of the articular fossa and condylar of the joints were performed using Mimics10.01 software. RESULTS AND CONCLUSION: The internal and external diameter of the mandibular fossa, head of mandible and neck of mandible were greater than the anterior and posterior diameter. The mandibular foramen was located at middle-posterior of the ramus of the mandible. The distance between head of the mandible and coronoid process was longer than the depth of the mandibular notch. There were no significant differences between two sides regarding all above-mentioned parameters. There are certain regular patterns in anatomy parameters of the temporomandibular joint. The study results will provide a reference for improving the design of artificial temporomandibular joint. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程  Figures and Tables | References | Related Articles | Metrics

Select

Trypsin plus type II collagenase digestion for isolation of nucleus pulposus cells: the optimal glucose concentration in complete medium Zhang Cun-xin, Ma Jin-feng, Wang De-chun 2016, 20 (20):  2899-2906.  doi: 10.3969/j.issn.2095-4344.2016.20.002 Abstract ( 439 )   PDF (5775KB) ( 372 )   Save BACKGROUND: Intervertebral disc degeneration is the pathological basis of degenerative spinal diseases. Studies on the influential factors of intervertebral disc degeneration contribute to the prevention and treatment of degenerative spinal disease. OBJECTIVE: To observe the growth and proliferation of nucleus pulposus cells isolated by trypsin plus type II collagenase digestion in complete medium with different glucose concentrations, exploring the optimal glucose concentration for growth of nucleus pulposus cells. METHODS: Nucleus pulposus cells isolated and cultured by trypsin plus type II collagenase digestion method were observed under an inverted microscope, and the cell number was counted. Morphology of nucleus pulposus cells was observed after hematoxylin-eosin staining and toluidine blue staining. Collagen type II immunoreactivity was detected by immunohistochemical staining combined with immunofluorescent staining. Nucleus pulposus cells were incubated in complete medium containing various glucose concentrations (0, 6.25, 12.5, 17.5, and 25 mmol/L) for 24 hours, and then cell proliferation and apoptosis were determined. RESULTS AND CONCLUSION: The stained nucleus pulposus cells showed polygonal and short spindle, with one or two nuclei. Cellular pseudopod appeared gradually and then became slim with increased passage numbers. The isolated and cultured nucleus pulposus cells positively expressed collagen type II and aggrecan Proliferative activity of nucleus pulposus cells cultured in medium with 17.5 mmol/L glucose was significantly higher than that in medium with 0 and 25 mmol/L glucose (P < 0.05 or P < 0.01). There was no significant difference in cell apoptosis between these groups except for 0 mmol/L glucose (P < 0.05). These results confirm that a large number of nucleus pulposus cells can be harvested by trypsin plus type II collagenase digestion and the optimal glucose concentration is 17.5 mmol/L. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Advanced glycation end products influence osteoclast-induced bone resorption Li Zi-qing1, Wang Hai-xing1, He Pei-heng1, Luo Guo-tian1, Xiao Yin-bo1, Huang Shuai1, Li Xing1, Sheng Pu-yi1, Li Chao-hong2, Xu Dong-liang1 2016, 20 (20):  2907-2914.  doi: 10.3969/j.issn.2095-4344.2016.20.003 Abstract ( 478 )   PDF (6782KB) ( 281 )   Save BACKGROUND: The effects of advanced glycation end products (AGEs) on osteoclast-induced bone resorption is controversial and the underlying mechanisms remain unclear. Most of the studies indicate that AGEs can enhance bone resorption, while some others show the opposite effects. OBJECTIVE: To investigate the effects of AGEs on osteoclast-induced inorganic matrix dissolution and organic component degradation and the underlying mechanisms. METHODS: RAW 264.7 cells were induced to generate osteoclasts, and AGEs (50-400 µg/mL) or control-bovine serum albumin (100 µg/mL) was added since the beginning of the induction. The effect of AGEs on bone resorption was evaluated by analyzing the area of resorption pits on the Osteo Assay Surface plates and the expression of cathepsin K. Furthermore, the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells, nuclei per osteoclasts and the expression of integrin ανβ3 were detected. RESULTS AND CONCLUSION: The area of resorption pits and expression of cathepsin K in AGEs groups were significantly decreased compared with the control group, and this inhibiting effect became more obvious with the increase of AGEs concentration. TRAP staining also showed that number of TRAP-positive multinucleated cells and nuclei per osteoclast were significantly reduced in an AGE dose-dependent manner. Quantitative PCR revealed that the expression of integrin ανβ3 decreased significantly with the extension of AGEs incubation time. These data indicate that AGEs can exert inhibitory effects on organic and inorganic matrix degradation induced by osteoclasts. The underlying mechanism may be involved in the inhibitory effects of AGEs on directed differentiation and cell fusion of osteoclast precursor cells, and migration and adhension of osteoclasts. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Biological properties of bone morphogenetic proteins and basic fibroblast growth factor in biological materials for repair of articular cartilage defect Dong Jun-bo1, 2 2016, 20 (20):  2915-2920.  doi: 10.3969/j.issn.2095-4344.2016.20.004 Abstract ( 362 )   PDF (3514KB) ( 639 )   Save BACKGROUND: Articular cartilage regeneration can be regulated by autocrine or paracrine secretion of various cytokines. OBJECTIVE: To analyze biological properties of bone morphogenetic proteins and basic fibroblast growth factor in biological materials for repair of articular cartilage defect. METHODS: Forty New Zealand white rabbits were used and equally randomized into four groups: fibrin, basic fibroblast growth factor, bone morphogenetic protein, and combined treatment (basic fibroblast growth factor combined with bone morphogenetic protein) groups, respectively. Bioactive scaffolds with fibrin, basic fibroblast growth factor, bone morphogenetic protein, and basic fibroblast growth factor combined with bone morphogenetic protein were injected to repair the articular cartilage defect. Therapeutic effect and biological properties of biological materials were compared. RESULTS AND CONCLUSION: (1) In the fibrin group, two rabbits appeared to have limps. In the basic fibroblast growth factor group hand function was limited in one rabbit. In the bone morphogenetic protein group, one had a limp and one was in a limitation of activity. In the combined treatment group, rabbits recovered well and showed no differences in the knee joint before and after surgery (P < 0.05). (2) General observation: In the combined treatment group, soft solution cartilage defects disappeared, and angiogenesis and cartilage were similar with normal tissues. In the bone morphogenetic protein group, fractured cartilage marginal existed and could not be closely integrated with normal cartilage. The presence of chondrocytes in the periphery of the defect was seen under light microscope. In the fibrin group, defect site and surrounding tissues healed. In the basic fibroblast growth factor group, defect was repaired, but not smooth. (3) Results of hematoxylin and eosin staining: In the fibrin group, the bone defect was not repaired, obvious depression surface was seen. In basic fibroblast growth factor group, repair of cartilage defect was obvious. There were a lot of chondrocytes. In the bone morphogenetic protein group, the bone defect was repaired; chondrocytes appeared, but irregular arrangement. In the combined treatment group, good bone defect repair and a large number of cartilage cells were seen. Taken together, biological materials with fibrin and basic fibroblast growth factor are ideal for repair of articular cartilage defect by promoting formation of cartilage by bone morphogenetic protein and enhancing chondrocyte proliferation by basic fibroblast growth factor. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 ORCID: 0000-0002-0170-1787(董君博) Figures and Tables | References | Related Articles | Metrics

Select

Effects of Lisfranc ligament on stability of tarsometatarsal joints: an anatomical and biomechanical study Su Bo-yuan, Sun Zhan-dong 2016, 20 (20):  2921-2926.  doi: 10.3969/j.issn.2095-4344.2016.20.005 Abstract ( 533 )   PDF (945KB) ( 256 )   Save BACKGROUND: Lisfranc ligament is the strongest one among three ligaments between the second metatarsal bone and the medial cuneiform, and it is critical for maintaining the stability between the second metatarsal bone and the medial cuneiform because Lisfranc ligament is three times stronger than the dorsal ligament. However, there have been no reports addressing the effects of Lisfranc ligament injury on the stability of tarsometatarsal joints. METHODS/DESIGN: An anatomical and biomechanical study was performed. Anatomical measurements were performed in the origin, terminal, path, length, width, and thickness of Lisfranc ligament utilizing cadaverous embalmed specimens. In the experiment on fresh-frozen cadaveric specimens, the displacement changes between the first and the second metatarsal base under different loading were measured with biomechanical analysis after the successively resected dorsal ligament, Lisfranc ligament, and plantar ligament were loaded, respectively, exploring the effect of Lisfranc ligament on stability of tarsometatarsal joints. DISCUSSION: This study provides the theoretical basis for the treatment of tarsometatarsal joint injury in the clinic through exploring the effects of Lisfranc ligament on stability of tarsometatarsal joints. The attention should be paid to Lisfranc ligament by the physicians when the tarsometatarsal joint injury occurs. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Effects of combined treatment using umbilical cord blood mesenchymal stem cell transplantation and paclitaxel on proliferation, apoptosis, and invasion of A2780 ovarian cancer cells Chen Feng-zhen, Yin Li-rong 2016, 20 (20):  2927-2932.  doi: 10.3969/j.issn.2095-4344.2016.20.006 Abstract ( 410 )   PDF (1074KB) ( 231 )   Save BACKGROUND: An intensive study on ovarian cancer A2780 cells contributes to modify treatment strategies targeting ovarian cancer stem cells and enhance survival rate of ovarian cancer patients. OBJECTIVE: To investigate the effects of umbilical cord blood mesenchymal stem cells (UC-MSCs) transplantation combined with paclitaxel on proliferation, apoptosis, and invasion of A2780 human ovarian cancer cells in vitro. METHODS: A2780 human ovarian cancer cells were assigned into three groups: blank control group (routine cell culture), UC-MSCs group (addition of suspension containing 1×106 UC-MSCs) and combined treatment group (combined addition of suspension containing 1×106 UC-MSCs and 250 μL of 1 μmol/L paclitaxel solution). CD133+ antigen expression, proliferation, apoptosis, and invasion were determined by immunofluorescent staining, flow cytometry, TUNEL staining, and Transwell chamber invasion assay, respectively. RESULTS AND CONCLUSON: CD133+ antigen expression in the UC-MSCs group and combined treatment group was significantly decreased compared with the blank control group (P < 0.05). Proliferation and invasion abilities were significantly decreased, while apoptosis cell number was significantly increased in the combined treatment group compared with the other two groups (P < 0.05). These findings indicate that the combined treatment of UC-MSCs transplantation and paclitaxel can inhibit proliferation and invasion and promote apoptosis of A2780 human ovarian cancer cells. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Implantation accuracy is not affected by head and angle deviation following computer aided design guide for repair of maxillary posterior tooth defects Yuan Zhi1, Chen Yi-hui2 2016, 20 (20):  2933-2939.  doi: 10.3969/j.issn.2095-4344.2016.20.007 Abstract ( 421 )   PDF (4000KB) ( 273 )   Save BACKGROUND: Maxillary posterior tooth defects can be repaired using the dental implant. However, implantation accuracy is not ensured by traditional plaster models used as implant template, which leads to the incidence of complications, and affects the repair effects. OBJECTIVE: To study application effects of surgical guide plates designed using the computer aided design (CAD)/computer aided manufacturing (CAM) technology for the repair of maxillary posterior tooth defects. METHODS: Implant treatment was performed in 30 patients with maxillary posterior tooth defects. Surgical guide plate was made using the CAD/CAM technology after CT scan of the jaw bone. The repair was conducted with guide plate assistant. The deviation between the virtual and actual implant position was measured after implantation. The clinical repair effects and periodontal tissues were observed during the 12-month follow-up. RESULTS AND CONCLUSION: Thirty-six CAD/CAM surgical guide plates in 30 patients were stabilized after implantation. Thirty-six implants were emplaced precisely with the assist of the surgical guide plates. There was no any problem during the process of implantation. The deviation of the head between the virtual and actual implant position was very small, which did not affect the accuracy of implant placement. Before and 6 and 12 months after the repair, gingival crevicular fluid, tumor necrosis factor-α, and sulcus bleeding index were not obviously changed in the patients. Our results suggest that the implant head deviation and angle deviation appear during the repair process of maxillary posterior tooth defects using CAD/CAM surgical guide for dental implants; however, the implantation accuracy is not affected. The repair effect is ideal and periodontal tissue is in good status after implantation. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Atorvastatin promotes implant osseointegration via the activation of Wnt/β-catenin signal pathway in osteoporotic rats Liang Yao-zhong1, Chen Shu2, Yang Yu-hao1, Lan Chun-hai1, Zhang Guo-wei1, Ji Zhi-sheng1, 2016, 20 (20):  2940-2948.  doi: 10.3969/j.issn.2095-4344.2016.20.008 Abstract ( 311 )   PDF (4916KB) ( 344 )   Save BACKGROUND: Atorvastatin has been shown to reduce bone loss and fracture, but its effects on implant osseointegration remain unknown. OBJECTIVE: To investigate the effects of atorvastatin on implant osseointegration in osteoporotic rats and the underlying mechanisms. METHODS: Forty-eight Sprague-Dawley rats were randomized into sham-surgery, ovariectomy, and atorvastatin (10 and 20 mg/kg per day) treatment groups, respectively. All rats received ovariectomy and implant surgery except those in the sham-surgery group. Bone mineral density of the lumbar vertebra, osseointegration ratio and pull-out strength of implants were measured after 12-week treatment. Levels of bone formation and resorption markers in osteoblasts treated with atorvastatin were determined by ELISA. Wnt pathway-related gene expression was detected by RT-PCR. RESULTS AND CONCLUSION: Bone mineral density, osseointegration ratio and pull-out strength of implants were significantly increased in 20 mg/kg per day of atorvastatin treatment group compared with ovariectomy group (P < 0.05). Levels of alkaline phosphatase, osteocalcin and osteoprotegerin were significantly increased in osteoblasts treated with atorvastatin in vitro (P < 0 .05), and the level of osteoclast differentiation factor RANKL was significantly inhibited (P < 0.05). Meanwhile, atorvastatin significantly promoted the mRNA expression of low-density lipoprotein associated protein 5 and β-catenin, and inhibited the mRNA expression of dickkopf Wnt signal pathway inhibitor 1 and sclerostin. Our results suggest that atorvastatin promotes implant osseointegration in osteoporotic rats by activating Wnt/β-catenin signal pathway. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Cardiac protection of prostacyclin secreted from endothelial progenitor cells against oxidative stress-induced apoptosis: verified by cardiac electrophysiological tests Zhang Jing, Ruan Lu, Kang Li, Cui Xiao-hai, Zhang Jia 2016, 20 (20):  2949-2956.  doi: 10.3969/j.issn.2095-4344.2016.20.009 Abstract ( 301 )   PDF (2041KB) ( 266 )   Save BACKGROUND: Prostacyclin (PGI2) and its analogs have been reported to prevent pressure overload-induced cardiac hypertrophy, and to reduce cardiac ischemia/reperfusion injury. However, clinical application of PGI2 is challenging due to its short half-life (< 2 minutes). Thus, we have generated PGI2 expressing rat endothelial progenitor cell strains (PGI2-EPCs) that constitutively secrete prostacyclin. OBJECTIVE: To investigate the protective effect of PGI2-EPCs against oxidative stress-induced cardiomyocyte injury. METHODS: Cultured H9c2 cells in vitro were assigned into four groups: H9c2 cells treated by H2O2 for 4 hours. H9c2 cells were pretreated by conditioned medium (collected form EPCs and PGI2-EPCs or collected form EPCs and PGI2-EPCs mixed with native EPCs) before the addition of H2O2. PBS instead of conditioned mediums served as negative control. The paracrine effect of PGI2-EPCs on in vitro angiogenesis of native EPCs was evaluated. MTT and Hoechst 33342 assays were used to examine the protective effect of conditioned medium on H2O2-induced rat embryonic cardiomyocyte apoptosis and cell viability. Finally, the effect of conditioned medium on the electric activities of adult cardiomyocytes was measured by whole-cell patch clamp techniques. RESULTS AND CONCLUSION: When native EPCs mixed with conditioned medium of PGI2-EPCs, the total length of tubes was significantly longer compared with those mixed with CM of EPC. Rat embryonic cardiomyocytes pretreated with conditioned medium of PGI2-EPCs significantly reduced H2O2-induced apoptosis and preserved cell viability compared with pretreatment with EPC-conditioned medium and without pretreatment (P < 0.01). Pretreatment of rat adult cardiomyocytes with conditioned medium of PGI2-EPCs abolished H2O2-induced early after depolarization and shortened H2O2-induced action potential duration prolongation (P < 0.01) towards baseline. Our findings indicate that PGI2-EPCs protect against oxidative stress-induced cardiomyocyte injury through paracrine action. This Study provides the groundwork for an innovative cell therapy approach to treat ischemic heart disease. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

EPAS1 gene rs6756667 polymorphism and aerobic exercise capacity of Tibetan athletes Jiang Li1, Yin Wei-yao2, Liu Jian3, Guo Hui4 2016, 20 (20):  2957-2963.  doi: 10.3969/j.issn.2095-4344.2016.20.010 Abstract ( 457 )   PDF (1231KB) ( 271 )   Save BACKGROUND: Emerging evidence shows that rs6756667 SNP EPAS1-A allele is beneficial for, however, the GG genotype is detrimental for adaptation to high-altitude hypoxia in Tibetan populations. OBJECTIVE: To compare the EPAS1 gene rs6756667 polymorphism between Tibetan elite endurance athletes and Tibetan non-physical education major students and analyze its relationship to aerobic exercise capacity. METHODS: Forty Tibetan athletes in Ganzi Tibetan middle and long distance athletes sports teams (athlete group) and forty Tibetan non-physical education major students in Ganzi (student group) were included in this study. The EPAS1 gene rs6756667 polymorphic loci were determined using PCR high-resolution melting analysis. The genetic polymorphism data from this study were compared with the data from previous studies. Increase values of bone mineral density in the pelvis, upper and lower limbs were determined by dual-energy X-ray absorptiometry. RESULTS AND CONCLUSION: There were significant differences in the genotype frequencies of EPAS1 rs6756667 AA, AG and GG between student group (50%, 47.5%, 2.5%) and athlete group (72.5%, 27.5%, 0; P < 0.05). The genotype frequency of GG was significantly increased in student group compared with athlete group (P < 0.05). There were differences in the frequencies of A allele and G allele between both groups (95% CI: 0.459-0.908, P=0.011). Bone mineral density in the pelvis, upper and lower limbs and whole body was significantly higher in student group than athlete group (P < 0.05). These findings suggest that EPAS1 gene rs6756667 polymorphism is related to aerobic capacity of Tibetan elite endurance athletes. rs6756667 SNP EPAS1-A allele is beneficial for, however, the GG genotype is detrimental for adaptation to high-altitude hypoxia in Tibetan athletes. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Mechanisms underlying the promotion of wound healing by bletilla carrying exogenous recombinant human epidermal growth factor Wang Xiao, Cui Ping, Wu Ran, Wang Bei, Qiu Shu-lin 2016, 20 (20):  2964-2971.  doi: 10.3969/j.issn.2095-4344.2016.20.011 Abstract ( 598 )   PDF (5316KB) ( 293 )   Save BACKGROUND: How to promote wound healing is always the research focus of the surgical physicians in the clinic. Recombinant human epidermal growth factor (rhEGF) can effectively promote wound healing. However, as a biological agent, it is easy to be decomposed under normal temperature. OBJECTIVE: To explore the mechanisms underlying the promotion of wound healing in the back of rabbits by bletilla carrying exogenous rhEGF. METHODS: Model rabbits with full-thickness skin defects in the back were treated with bletilla carrying exogenous rhEGF (combined treatment group), bletilla, rhEGF, or saline (control group). RESULTS AND CONCLUSION: The time of wound healing was the shortest (P < 0.05) and the wound healing rate was the highest (P < 0.05) in the combined treatment group. On postoperative days 3 and 10, newly formed granulation tissue, capillaries, and collagenous fibers showed by hematoxylin-eosin staining staining and Masson staining and the strongest immunoreactivity of vascular endothelial growth factor determined by immunohistochemical staining were found in the combined treatment group (P < 0.05). These findings confirm that bletilla carrying exogenous rhEGF promotes wound healing by accelerating the forming of granulation tissue, new-born capillaries, and collagenous fibers, and the effects are superior to either of them alone. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Acellular embryoid bodies in mice: preparation and effect of promoting differentiation of Lewis lung carcinoma cells Lv Wei-dong, Cai Lin, Zhang Jia-dong, Lei Guang-yan, Liu Zhi-gang, Zhang Xin-wei, Lu Jian-rong 2016, 20 (20):  2972-2978.  doi: 10.3969/j.issn.2095-4344.2016.20.012 Abstract ( 308 )   PDF (5921KB) ( 267 )   Save BACKGROUND: Co-culture with embryonic stem cells or embryonic tissues can induce differentiation of carcinoma cells into normal epithelial cells or decrease malignancy of carcinoma cells. Acellular embryoid bodies retain the structure and important cytokines of embryonic tissues. OBJECTIVE: To prepare acellular embryoid bodies from mouse embryonic stem cells and to investigate their effects on differentiation of mouse Lewis lung carcinoma cells at three-dimensional culture in vitro. METHODS: Mouse embryonic stem cells (D3) were dynamically cultured for 7 days to produce embryoid bodies followed by decellularization with 0.1% sodium dodecyl sulfate. Mouse Lewis lung carcinoma cells were co-cultured with acellular embryoid bodies as test group or cultured in three-dimensional matrigel medium for 7 days as control group, respectively. Cell proliferation and expression of E-cadherin were detected by immunohistochemical staining and western blot assay, respectively. In addition, mRNA expressions of Slug and E-cadherin were observed using RT-PCR technology. RESULTS AND CONCLUSION: Uniform mouse embryoid bodies were successfully prepared, and were completely decellularized with sodium dodecyl sulfate. After 7-day three-dimensional matrigel culture, in the control group, multicellular tumor spheroids were formed, accompanied by a higher Ki67 positive rate; Lewis lung carcinoma cells in the test group were repopulated in the acellular embryoid bodies showing significantly lower Ki67 positive rate. Compared with the control group, the absorbance of Paxillin in the test group was significantly smaller, and the absorbance of E-cadherin was significantly higher (P < 0.05). Besides, mRNA expressions of Slug and E-cadherin were significantly decreased and increased in the test group compared with the control group, respectively (P < 0.05). These findings indicate that the acellular embryoid bodies can promote differentiation of mouse Lewis lung carcinoma cells in three-dimensional culture in vitro. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松； ORCID: 组织工程0000-0003-2685-4218(吕卫东) Figures and Tables | References | Related Articles | Metrics

Select

Lentivirus-induced knockdown of low density lipoprotein receptor-related protein 1 aggravates cartilage damage in a rat model of osteoarthritis Yang Er-ping1, Peng Fei2, Liang Jie1, Du Yuan-li1 2016, 20 (20):  2979-2984.  doi: 10.3969/j.issn.2095-4344.2016.20.013 Abstract ( 291 )   PDF (4271KB) ( 334 )   Save BACKGROUND: Emerging evidence demonstrates that low density lipoprotein receptor-related protein 1 (LRP1) is involved in lipid metabolism and regulation of inflammatory reaction. OBJECTIVE: To explore the effect of lentivirus-induced knockdown of low density lipoprotein receptor-related protein 1 on cartilage damage and matrix metalloproteinase 13 in a rat model of osteoarthritis, so as to assess the role of low density lipoprotein receptor-related protein 1 in the pathogenesis of osteoarthritis. METHODS: Sixty-four Sprague-Dawley rats were included and ramdomly divided into four groups (n=16 for each): negative control group, no surgery; sham-surgery group, only the articular cavity of the knee was exposed; osteoarthritis plus shLRP1 group, rat osteoarthritis models were established by cutting anterior cruciate ligament and removing the medial meniscus partly followed by an intra-articular injection of lentivirus-mediated siRNA at 2 days after surgery, once a week for 2 consecutive weeks; osteoarthritis group, an intra-articular injection of the negative control lentivirus was performed after surgery. Rats in the four groups started running on the self-made electric treadmill from 5 days after modeling, 30 minutes per day, totally 500 meters. Cartilage damage and matrix metalloproteinase 13 expression in cartilage tissues were determined at 2, 4, 6 weeks after surgery. RESULTS AND CONCLUSION: Gross and pathological observations showed that lentivirus-induced knockdown of low density lipoprotein receptor-related protein 1 aggravated cartilage damage in the rat model of osteoarthritis. At 6 weeks after surgery, Mankin’s score and matrix metalloproteinase 13 expression in the cartilage tissues in osteoarthritis plus shLRP1 group were significantly increased compared with other three groups (P < 0.05). These findings indicate that a simulation model of osteoarthritis is developed by cutting anterior cruciate ligament and removing the medial meniscus partly combined with running on the treadmill. Lentivirus-induced knockdown of LRP1 aggravates cartilage damage in a rat model of osteoarthritis 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Changes of transcutaneous oxygen pressure and transcutaneous carbon dioxide pressure during altitude training Zhu Huan1, Gao Bing-hong2 2016, 20 (20):  2985-2991.  doi: 10.3969/j.issn.2095-4344.2016.20.014 Abstract ( 424 )   PDF (1145KB) ( 291 )   Save BACKGROUND: Generally, higher transcutaneous oxygen pressure indicates stronger aerobic fitness. Decreased transcutaneous oxygen pressure and increased transcutaneous carbon dioxide pressure reflect severe fatigue, muscle hypoxia, and poor function state. OBJECTIVE: To observe changes in transcutaneous oxygen pressure and transcutaneous carbon dioxide pressure in biceps brachii of excellent male rowing athletes during 6-week altitude training, and analyze relationship of the changes to training load. METHODS: The training contains three stages, i.e., before, during and after altitude training. Transcutaneous oxygen pressure and transcutaneous carbon dioxide pressure were determined using PeriFlux System 5000 laser Doppler flowmetry in athletes in the resting-fasting state in the morning. Lactic acid level in athletes was determined using 6-grade load test 1 week before altitude training and 2 weeks after the end of altitude training. RESULTS AND CONCLUSION: Transcutaneous oxygen pressure and the ratio of transcutaneous oxygen pressure to transcutaneous carbon dioxide pressure showed the W-shape change, and transcutaneous carbon dioxide pressure showed the V-shape change. These results suggest that the arrangement of the altitude training program is suitable, namely “reserve physical fitness at the plain stage, add amount and enhance degrees of altitude training load at the early and late stages”, and it can provide a better training load structure model for the post-altitude training. Transcutaneous oxygen pressure and transcutaneous carbon dioxide pressure, in particular the ratio of them, can be used to evaluate the degree of exercise load that athletes receive. At the same time, capillaries oxygen can be quantified by testing transcutaneous oxygen pressure. Transcutaneous oxygen pressure is recommended as a noninvasive index to monitor changes in aerobic capacity of athletes. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Application of three-dimensional scanning and measuring techniques in the diagnosis and treatment of mandibular prognathism Ding Yu-de, Yang Bin 2016, 20 (20):  2992-2999.  doi: 10.3969/j.issn.2095-4344.2016.20.015 Abstract ( 327 )   PDF (994KB) ( 262 )   Save BACKGROUND: A classical orthognathic scheme for mandibular prognathism includes preoperative cephalometric analysis, acquiring modulus, facebow transfer, model surgery, making occlusion plate. This process is cumbersome, complex, and prone to have bias. Moreover, orthognathic effects are difficult to be predicted, which is a major difficulty in doctor-patient communication. OBJECTIVE: To summarize and analyze the use of three-dimensional scanning techniques in the treatment and diagnosis of mandibular prognathism, including principle, application and relative merits, thus providing reference for applying to the clinic. METHODS: Papers addressing the use of three-dimensional scanning techniques in the diagnosis and treatment of mandibular prognathism were retrieved by computer in Wanfang and PubMed databases with the key words of “three-dimensional scanning, class III, orthognathic, mandibular prognathism” in Chinese and English, respectively. A total of 48 papers were included for review. RESULTS AND CONCLUSION: Compared with the traditional technology, the three-dimensional scanning technology has high accuracy and efficiency in the diagnosis and treatment of mandibular prognathism, and can directly capture the 3D geometry of objects for modeling, collection and measurement of biological data, which provides a reliable monitoring method for postoperative assessment and follow-up. Compared with the CT, the three-dimensional scanning technology cannot show the internal structure and separate hard and soft tissues, which should be reasonably chosen according to practical data. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 ORCID: 0000-0002-6535-4710(丁榆德) Figures and Tables | References | Related Articles | Metrics

Select

Efficacy of traditional Chinese medicine therapy and hyaluronic acid for knee osteoarthritis: a network Meta-analysis Liu Yu1, Ai Jin-wei1, 2, Liu Ying2, Li De-sheng1, Pei Bin1, 2 2016, 20 (20):  3000-3011.  doi: 10.3969/j.issn.2095-4344.2016.20.016 Abstract ( 436 )   PDF (2932KB) ( 553 )   Save BACKGROUND: Comparative study results between the efficacies of traditional Chinese medicine (TCM) therapy with Western medicine therapy in the treatment of knee osteoarthritis remain controversial. Currently, meta-analysis studies mainly focus on comparison of the efficacy between TCM and Western medicine, but lack investigations among TCM therapies. It makes us difficult to fully understand the efficacy of TCM therapy for knee osteoarthritis. OBJECTIVE: To compare the efficacies of TCM therapy with intra-articular injection of hyaluronic acid for knee osteoarthritis using a network Meta-analysis method. METHODS: PubMed, The Cochrane Library (tissue 10, 2015), EMbase, CNKI, CBM, VIP, Wangfang databases were searched up to October 20, 2015 for relevant randomized controlled trials addressing the efficacies of TCM therapy versus intra-articular injection of hyaluronic acid for knee osteoarthritis. Two reviewers independently selected the studies, extracted information, and assessed the quality of included trials. Data extraction from eligible studies was pooled and meta-analyzed using Stata12.0 and WinBUGS1.4.3 softwares. Odd ratios with their 95% confidence interval were estimated as effect size between treatments. RESULTS AND CONCLUSION: A total of 59 randomized controlled trials involving 6 155 patients with knee osteoarthritis and 12 treatment strategies were included. The results of network meta-analysis showed that TCM comprehensive therapy was superior to the TCM monotherapy and intra-articular injection of hyaluronic acid. Among these therapies, the efficacy of needle scalpel combined with acupuncture is optimal. TCM comprehensive therapies may be the best choice to treat patients with knee osteoarthritis of Kellgren-Lawrence II-III. More large-scale and well-designed randomized controlled trials are still warranted due to the limitation of the present study. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Ibandronate for postmenopausal osteoporosis: a Meta-analysis Liu Lei, Tao Sheng-xiang 2016, 20 (20):  3012-3021.  doi: 10.3969/j.issn.2095-4344.2016.20.017 Abstract ( 557 )   PDF (1828KB) ( 257 )   Save BACKGROUND: Alendronate has a marked enhancing effect on bone mineral density. Ibandronate is the newest (the third generation) bisphosphonates. Evidence-based medicine data on the therapeutic effects of both two drugs remain lacking because of different administration routes and time. OBJECTIVE: To compare the therapeutic effects of ibandronate and alendronate on postmenopausal osteoporosis. METHODS: A computer-based online search was conducted in CNKI, PubMed, CECDB, BMA, VIP, CQVIP, CBM, EMbase, ASP, The Cochrane Library, and EMCC databases from August 1999 to August 2015 to screen the relevant randomized controlled trials. Meta-analysis was performed using Stata/SE version 12.0 software by extracting data from the relevant articles. RESULTS AND CONCLUSION: A total of 10 randomized controlled trials involving 725 patients (ibandronate and alendronate for 379 and 346 patients, respectively) were included. The meta-analysis results showed that bone mineral density was significantly increased (P=0.174, I2= 29.4%, WMD=0.03, 95%CI (0.01-0.04)) at 6 months after treatment and incidence rate of fracture was significantly decreased (P=5.810, I2=0%, OR=0.32, 95%CI: (0.10-1.00)) during 1-year treatment with ibandronate compared with alendronate treatment. However, no significant differences were found between two drug treatments in lumbar spine bone mineral density at 1 year after treatment and the incidence rate of fracture during 6-month treatment. Six-month treatment of ibandronate can improve bone mineral density and reduce the incidence rate of fracture of patients with postmenopausal osteoporosis. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

All-arthroscopic rotator cuff repair versus mini-open for the treatment of rotator cuff injury: a Meta-analysis Wang Yi, Zhao Qi-chun 2016, 20 (20):  3022-3031.  doi: 10.3969/j.issn.2095-4344.2016.20.018 Abstract ( 422 )   PDF (1489KB) ( 282 )   Save BACKGROUND: There are controversies about the efficacy of all-arthroscopic rotator cuff repair versus mini-open for the treatment of rotator cuff injury. OBJECTIVE: To evaluate the efficacy of all-arthroscopic rotator cuff repair versus mini-open for the treatment of rotator cuff injury by conducting a meta-analysis. METHODS: A computer-based online search was conducted in PubMed, Embase, Cochrane Library and CBM databases from January 1966 to November 2015 to screen the relevant articles using the key words of “rotator cuff, arthroscopy, mini-open”. Meta-analysis was performed using Revman5.3 software. RESULTS AND CONCLUSION: A total of 11 studies, including 6 randomized controlled trials and 5 cohort studies, were selected. The meta-analysis results showed that there were no significant differences in the function and strength of the shoulder joint, pain, motor range, recurrence of rotator cuff avulsion, the incidence rate of ankylosis between both two groups (P > 0.05). These results suggest that the efficacy of all- arthroscopic rotator cuff repair does not differ from those of mini-open for the treatment of rotator cuff injury. However, arthroscopic rotator cuff repair induces less soft tissue injury and early incision pain, but better function recovery. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Preserving-remnant versus removing-remnant for arthroscopic anterior cruciate ligament reconstruction: a Meta-analysis Zhang Lei1, Xie Chong-xin2, Bai Jing-ping1 2016, 20 (20):  3032-3040.  doi: 10.3969/j.issn.2095-4344.2016.20.019 Abstract ( 334 )   PDF (1223KB) ( 276 )   Save BACKGROUND: There are controversies about preserving the remnant in the anterior cruciate ligament reconstruction procedure because of its uncertain therapeutic effects. OBJECTIVE: To compare the efficacy and safety of preserving-remnant with removing-remnant for arthroscopic anterior cruciate ligament reconstruction using a meta-analysis. METHODS: A computer-based online search was conducted in PubMed, Embase, the Cochrane Library, CDSR, CBM, and CNKI databases by using the English key words of “anterior cruciate ligament AND remnant (OR stump) AND randomized controlled trial (RCT) OR quasi-RCT” and the Chinese key words of “anterior cruciate ligament reconstruction, preserving-remnant, removing-remnant” to screen the relevant articles published from 1995 to July 2015. Meta-analysis was performed using Review Manager 5.3 software. RESULTS AND CONCLUSION: A total of 13 randomized controlled trials were included. The meta-analysis results showed that there were no statistically significant differences in KT1000/2000 scores (OR=-0.28, 95%CI: -0.76-0.20, P=0.25), the good rate of synoveal coverage (OR =-0.30, 95%CI: -0.30-0.90, P=0.32), and the incidence of cyclops leions (OR=0.87, 95%CI: 0.63-2.90, P=0.44). Postoperative Lysholm scores (OR=2.45, 95%CI: 0.52-4.39, P=0.01), proprioceptive function (OR=-1.72, 95%CI: -3.32 to 0.13, P=0.03), tunnel enlargement (OR=-0.66, 95%CI: -1.08 to -0.23, P=0.002) in preserving-remnant were superior to removing-remnant for arthroscopic anterior cruciate ligament reconstruction. These results suggest that both preserving-remnant and removing-remnant for arthroscopic anterior cruciate ligament reconstruction can obtain satisfactory antero-posterior stability of the knee. Preserving-remnant exhibits superiority in post-operative scores of the knee, proprioceptive function, tunnel enlargement. Further high-quality randomized controlled trials are warranted because of some low-quality studies and the existing biases. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics