Lentivirus-induced knockdown of low density lipoprotein receptor-related protein 1 aggravates cartilage damage in a rat model of osteoarthritis

doi:10.3969/j.issn.2095-4344.2016.20.013

Abstract

Abstract:

BACKGROUND: Emerging evidence demonstrates that low density lipoprotein receptor-related protein 1 (LRP1) is involved in lipid metabolism and regulation of inflammatory reaction.
OBJECTIVE: To explore the effect of lentivirus-induced knockdown of low density lipoprotein receptor-related protein 1 on cartilage damage and matrix metalloproteinase 13 in a rat model of osteoarthritis, so as to assess the role of low density lipoprotein receptor-related protein 1 in the pathogenesis of osteoarthritis.
METHODS: Sixty-four Sprague-Dawley rats were included and ramdomly divided into four groups (n=16 for each): negative control group, no surgery; sham-surgery group, only the articular cavity of the knee was exposed; osteoarthritis plus shLRP1 group, rat osteoarthritis models were established by cutting anterior cruciate ligament and removing the medial meniscus partly followed by an intra-articular injection of lentivirus-mediated siRNA at 2 days after surgery, once a week for 2 consecutive weeks; osteoarthritis group, an intra-articular injection of the negative control lentivirus was performed after surgery. Rats in the four groups started running on the self-made electric treadmill from 5 days after modeling, 30 minutes per day, totally 500 meters. Cartilage damage and matrix metalloproteinase 13 expression in cartilage tissues were determined at 2, 4, 6 weeks after surgery.
RESULTS AND CONCLUSION: Gross and pathological observations showed that lentivirus-induced knockdown of low density lipoprotein receptor-related protein 1 aggravated cartilage damage in the rat model of osteoarthritis. At 6 weeks after surgery, Mankin’s score and matrix metalloproteinase 13 expression in the cartilage tissues in osteoarthritis plus shLRP1 group were significantly increased compared with other three groups (P < 0.05). These findings indicate that a simulation model of osteoarthritis is developed by cutting anterior cruciate ligament and removing the medial meniscus partly combined with running on the treadmill. Lentivirus-induced knockdown of LRP1 aggravates cartilage damage in a rat model of osteoarthritis

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Lentivirus, Anterior Cruciate Ligament, Tissue Engineering

CLC Number:

R318

Yang Er-ping1, Peng Fei2, Liang Jie1, Du Yuan-li1. Lentivirus-induced knockdown of low density lipoprotein receptor-related protein 1 aggravates cartilage damage in a rat model of osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(20): 2979-2984.

Figures/Tables 1

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