Establishment of a rabbit model of oral soft tissue defects

doi:10.3969/j.issn.2095-4344.2016.18.015

Abstract

Abstract:

BACKGROUND: In clinical, the research on the method and the material of the soft tissue defect of the operation area has been in depth. It will have a positive impact on our exploration and research in this field through the establishment of reliable experimental animal oral soft tissue defect model.

OBJECTIVE: To establish a rabbit model of oral soft tissue defects for oral treatment of soft tissue defects in-depth study.

METHODS: Eighteen 3-month-old male New Zealand rabbits were enrolled. A tissue ring cutting drill of 5 mm diameter was used to prepare round full-thickness soft tissue defects in the front part of the hard palate and, respectively, from the back of the maxillary incisors, about 2 mm from the hard palate mucosal edge. Morphology and histology were observed at 3, 7, 14, 21, 28 and 56 days after model establishment.
RESULTS AND CONCLUSION: (1) Morphological observation: After 3 and 7 days, the wound’s inflammatory reaction was obvious. As the time goes, the inflammatory response subsided, the wound gradually healed. Scar formation was observed at 21, 28 and 56 days after surgery. (2) Histological observation: 3 and 7 days after injury, many inflammatory cells were infiltrating, and tissue necrosis area was larger. At 7 days after surgery, with the extensive formation of connective tissue proliferation and new blood capillaries, the wound gradually shaping completely. (3) Results indicated that a rabbit model of oral soft tissue defects was successfully established, which was in line with the physical laws of wound healing and the characteristics of human oral soft tissue defects healing.
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Models, Animal, Mouth

Yang Qin-qiu, Dong Lu, Xiao Qiong, Chen Hong-liang, Sun Yong. Establishment of a rabbit model of oral soft tissue defects[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(18): 2684-2689.

Figures/Tables 3

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