Vitreous cavity injection with erythropoietin protects optic nerve in a rat model of diabetic retinopathy

doi:10.3969/j.issn.2095-4344.2016.18.016

Abstract

Abstract:

BACKGROUND: Diabetes can lead to many complications. Of them, retinopathy is a common type. To explore the effect of erythropoietin in diabetic retinopathy, it is necessary to establish an animal model of similar pathologic features and high reproducibility in clinical retinal neovascularization.

OBJECTIVE: To investigate the protective effect of vitreous cavity injection of erythropoietin on optic nerve of rats with diabetic retinopathy.

METHODS: Rat models of diabetic retinopathy were established and injected with erythropoietin through vitreous space for 14 consecutive days. Results were compared between the model and normal control groups.

RESULTS AND CONCLUSION: (1) Transmission electron microscope: compared with the model group, retinal ganglion cell nucleus presented uniform red; cell membrane was intact; endoplasmic reticulum exhibited slight swelling. A small number of vacuoles were found in mitochondria around the nuclei. (2) Western blot assay and RT-PCR: compared with the model group, Caspase-3 and Bax protein expression was lower in the erythropoietin group (P < 0.05). Caspase-3 protein and Bcl-2 protein expression was significantly higher in the erythropoietin group than in the model group (P < 0.05). Significant differences in nuclear factor κB and Survivn gene expression were detected between the model and erythropoietin groups (P < 0.05). (3) Results verified that vitreous cavity injection of erythropoietin had neuroprotective effect on rats with diabetic retinopathy, which may be associated with the inhibitory effect on apoptosis.
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Models, Animal, Diabetes Mellitus

Zhang Yong-hong, Zhang Xiao-mei, Wei Xiao-yi, Tang Liu-ping, Liu Dai-hua, Ling Yu. Vitreous cavity injection with erythropoietin protects optic nerve in a rat model of diabetic retinopathy[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(18): 2690-2696.

Figures/Tables 5

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