Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (18): 2622-2627.doi: 10.3969/j.issn.2095-4344.2016.18.006

Previous Articles     Next Articles

Effect of Jisuikang on Nogo-66 receptor NgR expression in rats with spinal cord injury

Guo Yang1, Ma Yong1, 2, Feng Cheng1, Pan Ya-lan1, Huang Gui-cheng1   

  1. 1Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China; 2Department of Traumatology & Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • Received:2016-02-05 Online:2016-04-29 Published:2016-04-29
  • Contact: Ma Yong, Professor, Doctoral supervisor, Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China; Department of Traumatology & Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • About author:Guo Yang, M.D., Assistant researcher, Institute of Traumatology & Orthopedics and Laboratory of New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
  • Supported by:

    the Natural Science Research Program Project of Jiangsu Province Universities, No. 15KJD360001; the National Natural Science Foundation of China, No.81573997; the Postgraduate Science Research and Innovation Project of Jiangsu Colleges, No. CXZZ12_0609; the Jiangsu Province Natural Science Foundation, No. BK2011180

PDF

846

Abstract:

BACKGROUND: Myelin sheath related inhibitors have been found to have great impact on microenvironment of axon regeneration. Traditional Chinese medicine is gradually becoming a research hotspot on improving microenvironment of nerve regeneration with its advantage on multiple factors and targets.

OBJECTIVE: To clarify the effect of Jisuikang on Nogo-66 receptor NgR expression after spinal cord injury. 
METHODS: 144 rats were randomly divided into six groups: sham surgery group, model group, prednisone group, high-, moderate- and low-dose Jisuikang groups (n=24). Animal models of spinal cord injury were established by the modified Allen’s method in the later five groups. Rats in the prednisone group were daily given 0.06 g/kg prednisone acetate by lavage, once a day. Rats in the high-, moderate- and low-dose Jisuikang groups were daily intragastrically given 12.5, 25 and 50 g/kg Jisuikang, once a day. Rats in the sham surgery and model groups were intragastrically daily given 20 mL of saline, once a day. Rats in each group were administered drugs until death.
RESULTS AND CONCLUSION: Compared with the model group, NgR protein and mRNA expression levels were significantly lower in the prednisone, moderate-and low-dose Jisuikang groups. These data suggested that Jisuikang can improve the recovery of neurological function after spinal cord injury and effectively inhibit NgR protein expression at the site of injury so as to suppress the microenvironment factors harmful to nerve regeneration and further improve the microenvironment of nerve regeneration.
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Drugs, Chinese Herbal, Spinal Cord Injuries, Axons, Tissue Engineering