Establishing a rabbit model of knee paralysis by the knee fixation with plaster casts and wire

doi:10.3969/j.issn.2095-4344.2016.18.003

Abstract

Abstract:

BACKGROUND: Currently, the researches on knee plaster immobilization paralysis animal models are popular in the world, but there are some insufficiencies with the knee paralysis animals, for example, poor animal selection, inappropriate plaster selection and pure gypsum instability, which affect the subsequent results.

OBJECTIVE: To establish a rabbit model of knee paralysis by the knee fixation with plaster casts and wire.

METHODS: A total of 20 New Zealand white rabbits were randomly divided into two groups. Models of right knee paralysis were established and fixed by plaster casts and wire. Normal controls were considered as the control group. At 8 weeks after fixation, right knee and pathologic histology were observed in the right knee.

RESULTS AND CONCLUSION: The knee joints were translucent, smooth, and shiny, without the cartilage defect in the control group, and the cartilaginous elasticity and rigidity were good, and the arrangement of chondrocytes was normal. There were no inflammatory infiltrations in the articular capsule of the normal rabbits. In the model group, the knee joint was matt and opaque. Articular cartilage became thin, and showed poor elasticity. The cartilages were degenerated. Articular surface was rough, and had defects. There were small amount of joint fluid orno joint fluid. Cartilage cells shrank or disappeared, and the number of cartilage cells reduced with degeneration and necrosis. Subchondral bone became sclerosis with trabecular bone of hyperplasia. The arrangement of cartilage cells was disordered, with hyalinization and synovial villous hypertrophy. Cell clustering phenomenon was seen. There were a large number of lymphocytes, plasma cells and neutrophil infiltration in cartilages. The typical pathological changes of the knee paralysis present in the rabbit cartilage. These results demonstrate that the rabbit model of knee paralysis can be successfully established by using fixation method with plaster casts and wire, which has the advantages of simple operation, strong fixation and no injury.
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Models, Animal, Osteoarthritis, Knee, Tissue Engineering

Bai Xue, Zhang Hong-ping, Liu Jun-chang, Wang Xin-jun, Sun Yun, Zhou Dong-dong. Establishing a rabbit model of knee paralysis by the knee fixation with plaster casts and wire[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(18): 2603-2608.

Figures/Tables 2

References

[1] 王金涛,周顺利,易锦,等.分期推拿治疗膝痹病69例[J].北京中医药,2010,29(12):927-928.

[2] 余庆阳,黄巍.膝骨关节炎从痹论治的病因与证候探讨[J].风湿病与关节炎,2015,4(3):40-43.

[3] 李坤,肖四旺,陈孟交,等.丹紫康膝冲剂对大鼠膝骨性关节炎软骨细胞凋亡的影响[J].湖南中医药大学学报,2014, 34(3):20-23.

[4] 欧云生,安洪.鼠骨关节炎动物模型建立的现状[J].中国比较医学杂志,2004,14(1):41-44.

[5] Guzman RE,Evans MG,Bore S,et al.Mono-iodoacetate- induced histologic changes in subchondrla bone and articular cartilage of rat femorotial joints: an animal model of osteoarthritis.Taxied Pathol.2003;31(6):619-624.

[6] Mahr S,Menard J,Krenn V,et al.Sexual dimorphism in the osteoarthritis of STR/ort mice may eh link to articulra cytokines.Ann Rheum Dis.2003;62(12): 1234-1237.

[7] Yamamoto H,Iwase N.Spontaneous osteoarthritis lesions in a new mutant strain of the mouse.J Exp Anim Sic.1998;47(2):131-135.

[8] Yamamoto H,Iwase N,Kohno M.Histopathological characterization of spontaneously developing osteoarthropathy in the BCBC/Y mouse established newly from B6C3F1 mice. ExpToxicol Pathol.1999; 51(1):15-20.

[9] Muraoka T,Hagino H,Okano T,et al.Role of subchondral bone in osteoarthritis development: a comparative study of two strains of guinea pigs with and without spontaneously occurring osteoarthritis. Arthritis Rheum.2007;56(10):3366-3374.

[10] 熊勇,张照庆,夏数数,等.改良伸直位固定法建立兔膝骨性关节炎模型的研究[J].湖北中医药大学学报,2010,12(5): 19-21.

[11] Muehleman C,Green J,Williams JM,et al.The effect of bone remodeling inhibition by zoledronic acid in an animal model of cartilage matrix damage.Osteoarthr Cartilage.2002;10(3): 226-233.

[12] Kikuchi T,Sakuta T,Yamaguchi T.Intra-articular injection of collagenase induces experimental osteoarthritis in mature rabbit.Osteoarthr Cartilage.1998;6(3):177-186.

[13] Bouchgua M,Alexander K,D`Anjou MA,et al.Use of routine elinical multimodality imaging in a rabbit model of osteoarthritis- -partⅠ.Osteoarthr Cartilage.2009; 17(12): 188-196.

[14] Díaz-Gallego L,Prieton JG,Coronel P,et al.Apoptosis and nitric oxide in an experimental model of osteoarthritis in rabbit after hyaluronic acid treatment. J Orthop Res.2005;23(6):1370-1376.

[15] Hulmes DJ,Marsden ME,Strachan RK,et al. Intra-aricular hyaluronate in experimental rabbit osteoarthritis can prevent changes in cartilage proteoglycan content.Osteoarthr Cartilage.2004; 12(3):232-238.

[16] 汪青春,沈培芝,王海彬.骨关节炎动物模型的建立及选择[J].中医正骨,1998,10(3):39-40.

[17] Videman T.Experimental osteoarthritis in the rabbit：comparison of different periods of repeated immobilization. Acta Orthop Scand.1982;53(3): 339-347.

[18] 胡炯,杜宁,代岭辉,等.建立膝骨性关节炎关节软骨缺损的动物模型的研究[J].中国中医骨伤科杂志,2009,17(11): 3-16.

[19] 李澄.SD大鼠膝关节骨性关节炎模型的建立及其病理特征[D].中山大学,2010.

[20] 周海蓉,肖振良.健骨胶囊对免骨性关节炎软骨及滑膜影响的实验研究[J].中国中医药科技,2008,15(5):337-338.

[21] 郭玉冬,王宸,魏波,等.骨关节炎动物模型研究进展[J].东南大学学报:医学版,2012, 31(5):652-656.

[22] 熊勇,彭锐,夏数数,等.兔膝骨性关节炎关节液中白细胞介素1β和肿瘤坏死因子α表达与艾灸的影响[J].中国组织工程研究与临床康复,2010,14(41):7700-7703.

[23] 钟远鸣,全迪,贺启荣,等.舒筋汤熏洗加被动功能锻炼治疗膝骨性关节炎的实验研究[J].广西中医药,2008,31(1): 42-46.

[24] 罗灿峤,莫木琼,钟觉民.不同的脱钙液在骨组织制片中的比较应用[J].中国实用医药,2011, 6(19):27-28.

[25] 程永.膝痹病经筋病机与针刺治疗探讨[J].实用中医药杂志,2012,28(8):695-696.

[26] 葛广勇,赵建宁,刘刚,等.膝骨性关节炎模型的分期特征[J].中国临床康复,2006,10(4): 47-49.

[27] Mankin HJ,Dorfman H,Lippiello L,et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips.Ⅱ.Correlation of morphology with biochemical and metabolic data.J Bone Surg Am.1971;53(3):523-537.

[28] 于娟,李莎莎,叶莹仪,等.关节腔内注射高乌甲素对兔膝骨性关节炎的影响[J].中国比较医学杂志,2013,23(8): 34-37.

[29] 黄少君,孙升云,徐梅,等.淫羊藿对早期兔膝骨性关节炎软骨的影响[J].暨南大学学报:自然科学与医学版, 2014, 35(3):273-278.

[30] 谢希,高洁生.骨关节炎动物模型研究进展[J].医学综述, 2005,11(1):67-69.

[31] 钱洁,邢雪松,梁军.大鼠膝关节骨性关节炎动物模型的两种实验方案[J].实验室研究与探索,2014,33(11):23-27.

许文娟.树脂石膏与传统石膏应用在四肢骨折患者的效果优劣分析[J].医学美学美容:中旬刊,2013,22(10): 55-56.