Effects of FABP-5 gene silencing on the biological characteristics of the cervical carcinoma cell line SiHa

doi:10.3969/j.issn.2095-4344.2016.15.013

Abstract

Abstract:

BACKGROUND: Cancer related genes and pathways play a critical role in formation and development of cancer.

OBJECTIVE: To investigate the silencing epidermal fatty acid binding protein 5 (FABP-5) by siRNA interference on biological characteristics of the cervical carcinoma cell line SiHa.

METHODS: Design and synthesis of siRNA interference sequence used to transiently transfect SiHa cells was performed according to FABP-5 mRNA coding sequence. mRNA and protein expressions of FABP-5 were detected by RT-PCR and western blot assay, respectively. Meanwhile, cell cycle, proliferation, invasion and apoptosis were detected by flow cytometry, cell counting bit-8 assay, Boyden chamber assay, and TUNEL staining, respectively.

RESULTS AND CONCLUSION: FABP-5 mRNA and protein expressions, cell growth speed, cell number at S phase (cell cycle was arrested at the G₀/G₁ phase) and cell invasion were significantly decreased, while the cell apoptosis was significantly increased in FABP-5 siRNA group compared with the negative control and blank control groups. Our findings indicate that the specific silencing FABP-5 gene expression by siRNA interference can inhibit SiHa cervical cell growth and proliferation, but accelerate cell apoptosis.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: RNA, Small Interfering, Fatty Acid-Binding Proteins, Uterine Cervical Neoplasms, Cell Proliferation, Apoptosis

Chen Feng-zhen, Yin Li-rong. Effects of FABP-5 gene silencing on the biological characteristics of the cervical carcinoma cell line SiHa[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(15): 2218-2224.

Figures/Tables 8

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