Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (15): 2184-2189.doi: 10.3969/j.issn.2095-4344.2016.15.008

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Effects of Glycyrrhiza flavonoids on expression of perilipin mRNA and protein in skeletal muscle of rats after exhaustive exercise

Wang Cheng-ke1, Mo Wei-bin2, 3, Pan Chang-hong2, Zhang Meng2, Huang Dong2   

  1. 1Department of Sports, Guangxi Normal University for Nationalities, Chongzuo 532200, Guangxi Zhuang Autonomous Region, China; 2Sport School of Guangxi Normal University, Guilin 541004, Guangxi Zhuang Autonomous Region, China; 3State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guilin 541004, Guangxi Zhuang Autonomous Region, China
  • Received:2016-02-04 Online:2016-04-08 Published:2016-04-08
  • Contact: Mo Wei-bin, Master, Experimentalist, Sports School of Guangxi Normal University, Guilin 541004, Guangxi Zhuang Autonomous Region, China; State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guilin 541004, Guangxi Zhuang Autonomous Region, China
  • About author:Wang Cheng-ke, Master, Lecturer, Department of Sports, Guangxi Normal University for Nationalities, Chongzuo 532200, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31460232, 21562006; the Natural Science Foundation of Guangxi, No. 2015GXNSFAA139186; State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China Open Projects Fund, No. CMEMR2012-B04

Abstract:

BACKGROUND: Glycyrrhiza flavonoids can protect muscle tissues against oxidative and inflammatory injuries.
OBJECTIVE: To explore the effects of Glycyrrhiza flavonoids on energy storing and release of adipose tissue by studying expressions of perilipin mRNA and protein in skeletal muscle tissues of rats after exhaustive exercise.
METHODS: Fifty male healthy Sprague-Dawley rats were used and equally randomized into five groups: quiet control, exercise alone (intragastric administration with saline), exercise combined with low-, moderate-, high-dose Glycyrrhiza flavonoids (intragastric administration with Glycyrrhiza flavonoids) groups, respectively. Perilipin mRNA and protein expressions in skeletal muscle tissues containing gastrocnemius muscle and musculi soleus were determined at 6 weeks after exhaustive exercise.
RESULTS AND CONCLUSION: Expression of perilipin mRNA in rat gastrocnemius muscle in quiet control group was significantly decreased compared with exercise alone and all combined intervention groups (P < 0.05 or P < 0.01). Protein expression of perilipin in exercise combined with moderate- or high-dose glycyrrhiza flavonoids group was significantly increased compared with quiet control group (P < 0.05). Expression of perilipin mRNA in rat musculi soleus was significantly decreased compared with exercise combined with moderate-dose glycyrrhiza flavonoids group (P < 0.05). These findings confirm that Glycyrrhiza flavonoids are benefit for improvement of aerobic metabolism capacity of gastrocnemius muscle through regulating lipolysis pathway.
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程

Key words: Flavones, Muscle Fibers, Skeletal, Adipose Tissue