Clinical application of autogenous adipose-derived stromal vascular fraction-assisted fat transplantation in the improvement of facial skin quality

doi:10.3969/j.issn.2095-4344.1631

Abstract

Abstract:

BACKGROUND: Many studies have shown that cell-assisted lipotransfer promotes the survival of fat implants, but there are few studies exploring stromal vascular fraction (SVF)-assisted fat transplantation for improving facial skin quality.
OBJECTIVE: To evaluate the clinical effect of autologous adipose-derived SVF-assisted autologous fat transplantation in the improvement of facial skin quality.
METHODS: From September 2016 to June 2018, in the Plastic and Cosmetic Center, the Affiliated Hospital of Xuzhou Medical University, 20 patients were recruited and then randomly divided into experimental group and control group, namely SVF-assisted fat transplantation and simple fat transplantation, respectively. A VISIA skin detector was used to detect facial skin quality of patients preoperatively and 6 months postoperatively, including spots, wrinkles, texture, pores, ultraviolet spots, brown spots, couperose skin and porphyrin.
RESULTS AND CONCLUSION: The effect of improvement in wrinkles and texture in the experimental group was better than that of the control group, and the difference was statistically significant (P < 0.05). In terms of spots, pores and couperose skin, the therapeutic effect in the experimental group was better than that in the control group, but the difference was not statistically significant (P > 0.05). There was no significant difference between the two groups in the improvement of ultraviolet spot and brown spots. The porphyrin was not affected by the subjects. To conclude, SVF can promote the effect of fat transplantation in the improvement of facial skin quality.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Adipose Tissue, Tissue Transplantation, Skin, Face, Tissue Engineering

CLC Number:

R459.9

Yin Yating, Zhang Aijun, Wang Hao, Wang Pingping, Li Jianhua, Wen Minmin, Jin Peisheng. Clinical application of autogenous adipose-derived stromal vascular fraction-assisted fat transplantation in the improvement of facial skin quality[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(9): 1428-1433.

Figures/Tables 2

2.4 基质血管组分形态及流式细胞鉴定结果通过酶消化法分离提取的基质血管组分，重悬后得到以脂肪干细胞为主的细胞悬液，见图2A，其中含有大量红细胞，因而外观呈红色。基质血管组分种植于DMEM/F12+体积分数为10%胎牛血清+1%青-链霉素的完全培养基中，24 h内约90%细胞贴壁，呈多角形和短梭形，48 h后逐渐伸展为长梭形，见图2B。传代纯化培养后，取第3代细胞经流式细胞仪检测为CD90+/CD105+/CD44+/CD34-/CD31-/CD106-细胞，符合脂肪干细胞表面标记物特征，见图2C。 2.5 基质血管组分细胞计数和活力上清采样培养5 d检测，无细菌、真菌生长。流式细胞仪计数各组分细胞浓度为(1-3)×1010 L-1，锥虫蓝检测细胞活力>90%。 2.6 患者面部VISIA皮肤检测结果见图3。治疗前后记录VISIA皮肤检测仪各指标百分位数值，以术后测量值减去术前测量值作为治疗效果改善值，见表1。治疗后，试验组和对照组患者皮肤质量在斑点、皱纹、纹理、毛孔、紫外线色斑、棕色斑、红色区方面较治疗前均有所改善。在皱纹、纹理方面，试验组治疗效果改善值高于对照组，差异有显著性意义(P=0.004，0.034)，表明试验组在皱纹和纹理方面的治疗效果优于对照组。在斑点、毛孔及红色区方面，试验组治疗效果改善值高于对照组，但差异无显著性意义(P > 0.05)。在紫外线色斑、棕色斑方面，两组治疗效果改善值差异也无显著性意义(P > 0.05)。紫质主要为寄生在毛囊口的细菌代谢产物，反映即刻皮肤的清洁程度，其结果不受研究对象的影响。 2.7 典型病例患者术前双侧面颊、颞部明显凹陷，面部呈长方形，鼻唇沟较深，口角、眼尾等皱纹明显，面部填充基质血管组分辅助脂肪移植后，整体轮廓变得圆润，法令纹及其他皱纹均变浅。面部软组织容量明显增加，皮肤质地改善明显，皮肤较术前有光泽，见图4。 2.8 患者满意度试验组与对照组患者满意度均>90%。 2.9 不良反应所有患者治疗后均未发生排异、感染、坏死、液化、钙化、面神经损伤等并发症。"

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