Endometrial stromal stem cells from early-pregnancy decidua differentiate into endometrial epithelial cells

doi:10.3969/j.issn.2095-4344.2016.14.018

Abstract

Abstract:

BACKGROUND: Decidua tissue is one of the most important sources of endometrial stem cells, from which stromal stem cells can differentiate into various kinds of cells under contain inductions.

OBJECTIVE: To observe the differentiation of endometrial stromal stem cells isolated from early-pregnancy decidua into endometrial epithelial cells.

METHODS: Endometrial stromal stem cells from early-pregnancy decidua were cultured, identified and directionally induced. Then induced cells were randomized into test group cultured in conditioned medium and control group cultured in normal culture medium containing fetal bovine serum. Expression of keratin was detected by immunofluorescence assay and western bolt assay in the two groups.

RESULTS AND CONCLUSION: The endometrial stromal stem cells from early-pregnancy decidua were successfully isolated and cultured, and presented with a S-shaped curve. Within 1-48 hours after inoculation, cultured cells were in the adaptation phase; then, the cells proliferated rapidly and began to grow logarithmically at 48 hours after culture; after 60 hours of culture, the cell growth rate decreased, with a steady rise in the cell curve. Results from the flow cytometry detection showed that the endometrial stromal stem cells could express CD73 and CD90, but not express CD34, CD45 and cytokeratin. Immunofluorescence findings showed the expression of keratin negative in the control group, and mostly positive in the test group. Besides, the relative expression of keratin in the test group was significantly higher than that in the control group (P < 0.05). These results suggest that the endometrial stromal stem cells can be isolated from the human decidua in early pregnancy, and differentiate into endometrial epithelial cells by directional induction.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Uterus, Stem Cells, Decidua, Tissue Engineering

Chen Bo. Endometrial stromal stem cells from early-pregnancy decidua differentiate into endometrial epithelial cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2098-2103.

Figures/Tables 4

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