Seminiferous capsule extract effect on the proliferation of spermatogonial stem cells

doi:10.3969/j.issn.2095-4344.2016.14.019

Abstract

Abstract:

BACKGROUND: Chinese herbs for kidney nourishment can promote the proliferation and differentiation of spermatogonial stem cells.

OBJECTIVE: To verify the effect of seminiferous capsule extract on spermatogonial stem cell proliferation.

METHODS: Spermatogonial stem cells were isolated from the testis of male mice and synchronized by serum-free medium followed by an addition of 10, 50, 100 mg/L seminiferous capsule extracts. After 24 hours of culture, viability, proliferation and cell cycle of spermatogonial stem cells were observed.

RESULTS AND CONCLUSION: Compared with the control group, seminiferous capsule extracts promoted the cell number, viability and proportion at S stage. The number of BrdU-labeled spermatogonial stem cells was increased significantly after intervention with seminiferous capsule extracts, especially at the concentration of 50 mg/L. These findings indicate that seminiferous capsule extracts can promote the proliferation and viability of spermatogonial stem cells.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Spermatozoa, Cell Proliferation, Tissue Engineering

Li Jun-tao, Zhang Pei-hai, Qu Xiao-wei, Li Zheng-sheng, Li Song-wei. Seminiferous capsule extract effect on the proliferation of spermatogonial stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2104-2109.

Figures/Tables 6

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