Bone marrow mesenchymal stem cell transplantation for gastric precancerous lesions in a rat model

doi:10.3969/j.issn.2095-4344.2016.14.014

Abstract

Abstract:

BACKGROUND: Precancerous lesions are a long-term development process in which many factors are involved. Bone marrow mesenchymal stem cells can repair tissue injury.

OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cell transplantation on gastric precancerous lesions in the rats.

METHODS: Thirty-six Wistar rats were randomly divided into control group, model group and transplantation group. Animal models of gastric precancerous lesions were established in the model and transplantation groups. Rats in the transplantation group were given 1 mL of CM-dil-labeled bone marrow mesenchymal stem cells (3×10⁶ cells) via the tail vein, once a week, totally three times. Rats in the model and control group were subjected to the tail vein injection of the same volume of normal saline. Then, rats were sacrificed 1 week after final injection, and pathohistological changes in rat gastric tissue sections were observed. The expression of vascular endothelial growth factor in the gastric mucosa and levels of serum cytokines were detected.

RESULTS AND CONCLUSION: The severity of gastric mucosal injury in the transplantation group was lighter than that of the model group. The expression of vascular endothelial growth factor was significantly higher in the transplantation group compared with the model and control groups (P < 0.05). The levels of serum interleukin-17 and interferon-γ were significantly higher in the model group than the transplantation group followed by the control group (both P < 0.05). Therefore, bone marrow mesenchymal stem cells can increase vascular permeability, reduce inflammation, block or ease the occurrence of precancerous lesions by up-regulating the expression of vascular endothelial growth factor in the gastric mucosa lesions and reducing the expression of interleukin-17 and interferon-γ.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Precancerous Conditions, Gastric Mucosa, Bone Marrow, Mesenchymal Stem Cell Transplantation, Vascular Endothelial Growth Factors, Tissue Engineering

Liu Hong-feng, Li Lu. Bone marrow mesenchymal stem cell transplantation for gastric precancerous lesions in a rat model[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2073-2079.

Figures/Tables 5

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