Umbilical cord blood stem cell transplantation for treatment of decompensated cirrhosis

doi:10.3969/j.issn.2095-4344.2016.14.015

Abstract

Abstract:

BACKGROUND: Umbilical cord blood stem cells can be differentiated into functional liver cells in vivo, instead of mature liver cells in the treatment of liver diseases.

OBJECTIVE: To observe the effect of umbilical cord blood stem cell transplantation on decompensated cirrhosis in rats.

METHODS: Twenty Sprague-Dawley rats were enrolled and given 40% carbon tetrachloride peanut oil to make decompensated cirrhosis models. Eight weeks after modeling, model rats were randomized into control and experimental groups (n=10 per group), and subjected to injection of umbilical cord blood stem cell suspension (2 mL, 1.0×10⁷/L) or cell culture medium (2 mL) via the tail vein, respectively. At 4 weeks after injection, levels of serum aspartate aminotransferase, alanine aminotransferase, albumin, total bilirubin, hyaluronic acid, laminin, procollagen III and type IV collagen were detected; rat liver tissues were sliced for pathological observation.

RESULTS AND CONCLUSION: Compared with the control group, the albumin level was significantly increased but the levels of serum aspartate aminotransferase, alanine aminotransferase, total bilirubin, hyaluronic acid, laminin, procollagen III and type IV collagen decreased significantly in the experimental group (P < 0.05). From the pathological view, severity of liver cirrhosis was lower in the experimental group than the control group. Therefore, umbilical cord blood stem cell transplantation can effectively restore the liver function of decompensated cirrhosis in rats.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Cord Blood Stem Cell Transplantation, Liver Cirrhosis, Tissue Engineering

Guo Xian-li, Liu Yue, Zhou Li-min, Hu Yu. Umbilical cord blood stem cell transplantation for treatment of decompensated cirrhosis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2080-2085.

Figures/Tables 5

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