In vitro activation of macrophages by decellularized extracellular matrix materials

doi:10.3969/j.issn.2095-4344.2015.47.026

Abstract

Abstract:

BACKGROUND: Because macrophages play an important role in the body’s inflammatory response, the detection of the impact of biological materials on the behavior of macrophages can assess the immunogenicity of materials.

OBJECTIVE: To analyze the activation effect of decellularized extracellular matrix materials on macrophages.

METHODS: The peritoneal macrophages of BALB/c mouse were obtained and cultured by dividing into five groups. Control group was simple cell culture group, experimental group 1 was acellular matrix membrane material directly contacting with macrophage for culture, experimental group 2 was fresh pericardial material directly contacting with the macrophage for culture, experimental group 3 was acellular matrix membrane material indirectly contacting with macrophages for culture, experimental group 4 was fresh pericardium material indirectly contacting with macrophages for culture. After 24 hours of culture, the secretion of nitric oxide and cytokines in cell culture supernatant was determined. After 48 hours of culture, the absorbance value was determined by MTT method and the toxicity grading was determined.

RESULTS AND CONCLUSION: The toxicity grading in experimental groups 1-4 was respectively grades 2, 4, 0, 2. The nitric oxide level in experimental groups 1 and 2 was higher than that in the control group (P < 0.05), and the nitric oxide level in the experimental group 2 was higher than that in the experimental group 1 (P < 0.05). There were no significant differences in interleukin-2, interleukin-4, interferon γ, interleukin-17A and interleukin-10 levels between these five groups. The interleukin-6 level in the experimental group 2 was higher than that in the control group (P < 0.05); The expression levels of tumor necrosis factors in experimental group 1, 2 and 4 were higher than those in the control group (P < 0.05), and experimental group 2 higher than the experimental group 1 (P < 0.05), experimental group 1 higher than the experimental group 4 (P < 0.05). These results show that acellular matrix material can activate macrophages in direct contact.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Extracellular Matrix, Inflammation, Macrophages, Tissue Engineering

Xu Bin, Huang Xiu-yan, Wei Xiao-hui, Zeng Yao-ying. In vitro activation of macrophages by decellularized extracellular matrix materials[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(47): 7687-7692.

Figures/Tables 3

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