Hemostatic mechanism of calcium alginate dressing

doi:10.3969/j.issn.2095-4344.2015.47.025

Abstract

Abstract:

BACKGROUND: In recent years, calcium alginate dressing has been widely used in surgical hemostasis, traumatic hemostasis, postoperative nasal hemostasis and puncture site hemostasis, etc.; however, there are few reports on their hemostatic mechanisms.
OBJECTIVE: To preliminarily study the hemostatic mechanism of calcium alginate dressing.
METHODS: Human anticoagulant blood was respectively dropped on sodium alginate dressing, nasopore dressing and medical cotton gauze. After 2 minutes, the interaction between materials and blood was observed at the room temperature using scanning electron microscopy. Calcium alginate dressing, nasopore dressing and medical cotton gauze were added in human red blood cell suspensions respectively. After 15 minutes, the interaction between materials and red blood cells was observed using scanning electron microscopy. The red blood cells were suspended by different concentrations (10, 5, 2.5 g/L) of alginate dressing extracts. The erythrocyte sedimentation rate was observed at different time points (30, 60, 120 minutes). Platelets rich plasma was incubated with different concentrations (10, 5, 2.5 g/L) of alginate dressing extract at 37 ℃, then CD62P positive platelet percentage was measured by flow cytometry after 10 minutes of incubation.
RESULTS AND CONCLUSION: Dense fibrin network was formed after calcium alginate dressing contacting with an anticoagulant. A large number of blood cells were recruited. There were only a small amount of red blood cells and platelets adhesion in the nasopore dressing and medical cotton gauze groups. After the calcium alginate dressing interacting with red blood cells, red blood cell deformability was visible, with a pseudopodia-like change. The red blood cell morphology was unchanged in the nasopore dressing and medical cotton gauze groups. The calcium alginate dressing extract dose-dependently and time-dependently increased the red blood cells aggregation, comparative differences between groups was statistically significant(P < 0.01). The calcium alginate dressing extract dose-dependently enhanced the CD62P positive platelet percentage, comparative differences between groups was statistically significant (P < 0.01). These results demonstrate that calcium alginate dressing promotes hemostasis and coagulation process by releasing of calcium ions, causing red blood cell aggregation and deformation and activating platelets.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biological Dressings, Blood Coagulation, Erythrocyte Aggregation, Platelet Activation, Tissue Engineering

Cui Fei-yan, Wang Bin, Wei Li, Wang Hai-tao, Chen Hao, Chu Xiao-xia, Wang Zhen-zhen, Yang Li-hua. Hemostatic mechanism of calcium alginate dressing[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(47): 7681-7686.

Figures/Tables 5

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