Effectiveness of mineralized gelatin electrostatic spinning on inducing periodontal tissue osteogenesis

doi:10.3969/j.issn.2095-4344.2015.47.007

Abstract

Abstract:

BACKGROUND: Currently, little is reported regarding the effectiveness of mineralized gelatin electrostatic spinning fibers on periodontal tissue osteogenic induction.

OBJECTIVE: To investigate the effect of mineralized gelatin electrostatic spinning on the proliferation and osteogenic differentiation of periodontal ligament fibroblasts.

METHODS: Human periodontal ligament fibroblasts were respectively compositely cultured with non-mineralized gelatin electrostatic spinning, gelatin electrostatic spinning after 1 day of nano-hydroxyapatite mineralization and gelatin electrostatic spinning after 5 days of nano-hydroxyapatite mineralization. Cell proliferation was determined at 1, 4, 7, 10 and 13 days of culture using MTT assay. Alkaline phosphatase activity of cells was determined at 1, 7, 14 days of culture using biochemical analyzer.

RESULTS AND CONCLUSION: At the 10^th day of culture, the periodontal ligament cells on the surface of membrane distributed uniformly, grew well, spread out as sheetsthe gelatin electrostatic spinning after nano-hydroxyapatite mineralization may promote the proliferation and osteogenic differentiation of periodontal ligament fibroblasts in a time-dependent manner.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 and secreted a large amount of extracellular matrix, linked closely with the materialsl; moreover, the effect was more obvious at 5 days of mineralization. Cell growth density and status were better than those in the non-mineralized gelatin electrostatic spinning membrane group. Cell proliferation and alkaline phosphatase activities at different time points: 5-day mineralized gelatin electrostatic spinning membrane group > 1-day mineralized gelatin electrostatic spinning membrane group > non-mineralized gelatin electrostatic spinning fibers group (all P < 0.05). These results demonstrate that

Key words: Gelatin, Durapatite, Fibroblasts, Tissue Engineering

Guo Rui-zheng, Wang Ya-li . Effectiveness of mineralized gelatin electrostatic spinning on inducing periodontal tissue osteogenesis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(47): 7583-7588.

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