Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (47): 7589-7596.doi: 10.3969/j.issn.2095-4344.2015.47.008

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Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold to construct tissue-engineered larynx-shaped cartilage

Sun An-ke1, Meng Qing-yan2, Li Wan-tong2, Liu Song-bo3 , Chen Wei4   

  1. 1 Department of Otolaryngology, General Hospital of Shenyang Military Area Command, Shenyang 110016, Liaoning Province, China
    2 Department of Burns and Aesthetic Medicine, General Hospital of Shenyang Military Area Command, Shenyang 110016, Liaoning Province, China
    3 Department of Microsurgery, General Hospital of Shenyang Military Area Command, Shenyang 110016, Liaoning Province, China
    4 Department of Experimental Medicine, General Hospital of Shenyang Military Area Command, Shenyang 110016, Liaoning Province, China
  • Received:2015-09-12 Online:2015-11-19 Published:2015-11-19
  • Contact: Sun An-ke, Department of Otolaryngology, General Hospital of Shenyang Military Area Command, Shenyang 110016, Liaoning Province, China
  • About author:Sun An-ke, M.D., Chief physician, Department of Otolaryngology, General Hospital of Shenyang Military Area Command, Shenyang 110016, Liaoning Province, China
  • Supported by:

    the Key Fund of General Hospital of Shenyang Military Area Command, No. zy2009z0019

Abstract:

BACKGROUND: The study of tissue-engineered cartilage with predetermined shaping and regeneration has provided novel ideas and techniques for repair of laryngeal cartilage erosion; however, due to the special natures of the morphology, location and function of laryngeal cartilage, tissue engineering research has not, to date, exhibited its full advantages in the reconstruction of laryngeal cartilage.

OBJECTIVE: To explore the feasibility of building tissue-engineered larynx-shaped cartilage using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHH) as a scaffold filled and encapsulated with pedicled myofascial flaps.
METHODS: Porous PHBHH was prepared and formed into a hollow like larynx-shape, and the cell PHBHH composites were cultured for 1 week in vitro prior to implantation in vivo. The cell-PHBHH composite was filled and encapsulated with myofascial flaps with the pedicle for in situ implantation in nine rabbits as experimental group. PHBHH scaffold with no chondrocytes was implanted alone in three rabbits as control group. Cartilage regeneration was assessed at 6, 12 and 18 weeks after surgery through morphological observation, histological and immunohistochemical detection.
RESULTS AND CONCLUSION: In the experimental group, the shape and porosity (> 90%) of the material were ideal, the cells exhibited good adhesion with the material and the blood supply within the myofascial flap with pedicle was rich for effective filling and encapsulation of the cell PHBHH composite. Tissue-engineered laryngeal cartilage with the hollow, semi-trumpet shape was ideally formed at 6 weeks after the surgery. Further maturation of the cartilage was observed at 12 and 18 weeks after the surgery. However, there was no cartilage tissue in the control group. This study shows that PHBHH is a suitable material for the formation of a hollow, semi-trumpet shape with good cellular compatibility. Myofascial flap filling and encapsulating can be used to build tissue-engineered laryngeal cartilage with a hollow, semi-trumpet shape.
中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Tissue Engineering, Larynx, Cartilage