Mechanical property of silver-loaded coralline hydroxyapatite bone in the repair of large segmental contaminative radial defects

doi:10.3969/j.issn.2095-4344.2015.47.005

Abstract

Abstract:

BACKGROUND: Scholars have made certain progress in the basic and clinical studies regarding antibacterial bone graft materials.
OBJECTIVE: To investigate the mechanical property of antimicrobial silver-loaded coral hydroxyapatite bone using in vitro mechanical experiments and to explore its ability to repair large segmental contaminative bone defects.
METHODS: Compression test and three-point bending test were used to evaluate the mechanical properties of silver-load coralline hydroxyapatite, coralline hydroxyapatite and coral. Thirty-six New Zealand white rabbits were selected and randomly divided into four groups to establish right-side large segment of contaminative radial bone defect models. Rabbits in three groups were implanted silver-load coralline hydroxyapatite, coralline hydroxyapatite and in situ autologous bone, and rabbits in the other group were not implanted any material (ascontrol). At 2, 6 and 10 weeks post-operation, the rabbits were sacrificed to take specimens. The repair of bone defects in each group was observed and compared by gross observation, radiographic examination and histological examination. The antimicrobial condition in each group was evaluated by bacteriological examination.
RESULTS AND CONCLUSION: There was no significant difference between the mechanical properties of silver-load coralline hydroxyapatite, coralline hydroxyapatite and coral. At the 10th week post-operation, X-ray and histological observation showed mature bone tissues in the silver-load coralline hydroxyapatite bone group. A large number of lacunae and mature bone cells were visible in bone tissues. Haversian system was visible. Most of the materials were degraded, and there was only a small amount of residual material. Partial recanalization was visible in bone marrow cavity. The repair effect of silver-load coralline hydroxyapatite bone group was similar with the autologous bone group, and better than the coralline hydroxyapatite group and the control group. Bacteriological examination showed that the silver-load coralline hydroxyapatite had good antibacterial ability. These results demonstrate that the silver-load coralline hydroxyapatite has good mechanical properties and antibacterial properties, which can be used to repair large segmental contaminative bone defects.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Durapatite, Silver, Biomechanics, Tissue Engineering

Zhang Yu, Yin Qing-shui, Zhang Yu. Mechanical property of silver-loaded coralline hydroxyapatite bone in the repair of large segmental contaminative radial defects[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(47): 7567-7574.

Figures/Tables 10

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