Corneal distribution of stromal cell-derived factor-1 and chemokine receptor-4 as homing factors

doi:10.3969/j.issn.2095-4344.2015.46.017

Abstract

Abstract:

BACKGROUND: Stromal cell-derived factor-1 (SDF-1) and chemokine receptor-4 (CXCR4) play an important role in stem cell homing during bone marrow, organ and tissue transplantation. But there are few reports on limbal stem cell transplantation and homing as well as the distribution and content of SDF-1/CXCR4 on the cornea.

OBJECTIVE: To detect the expression levels of SDF-1/CXCR4 mRNA in rat corneal tissue by fluorescence quantitative PCR.

METHODS: The normal rat cornea was taken completely, and subdivided into central cornea, peripheral corneal and limbal area. Fluorescence quantitative PCR method was employed to detect SDF-1/CXCR4 mRNA levels at corresponding positions.

RESULTS AND CONCLUSION: The expression levels of SDF-1 and CXCR4 mRNA were 0.213±0.015 and 0.189±0.02 in the central cornea, 0.529±0.077 and 0.285±0.015 in the peripheral cornea, 0.666±0.069 and 0.258±0.067 in the limbal area, respectively. These data show that there are significant differences in the expression levels of SDF-1 and CXCR4 mRNA in the central cornea, peripheral corneal and limbal area, and these differences exert an important role in cell homing and migration during limbal stem cell transplantation for treatment of limbal stem cell deficiency.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Cornea, Receptors, CXCR4, Stem Cells

Zhang Wei, Huang Wen-zhi, Zhang Yu-ping. Corneal distribution of stromal cell-derived factor-1 and chemokine receptor-4 as homing factors [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(46): 7466-7471.

Figures/Tables 3

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